cloningandcharacterizationofgenesencodedindtdpdmycaminosebiosyntheticpathwayfromamidecamycinproducingstrainstreptomycesmycarofaciens

	cloningandcharacterizationofgenesencodedindtdpdmycaminosebiosyntheticpathwayfromamidecamycinproducingstrainstreptomycesmycarofaciens
	Cong Lina 2; Wolfgang PIEPERSBERG 1
刊名	actabiochimicaetbiophysicasinica
	2007-01-01
卷号	39 期号:3 页码:187
ISSN号	1672-9145
英文摘要	Two subclusters from Streptomyces mycarofaciens, a midecamycin producer, were cloned and partially sequenced. One region was located at the 5' end of the mid polyketide synthase (PKS) genes and contained the genes midA, midB and midC. The other region was at the 3' end of the PKS genes and contained midK, midI and midH. Analysis of the nucleotide sequence revealed that these genes encode dTDP-glucose synthase (midA), dTDP-glucose dehydratase (midB), aminotransferase (midC), methyltransferase (midK), glycosyltransferase (midI) and an assistant gene (midH). All of these genes are involved in the biosynthesis of dTDP-D-mycaminose, the first deoxysugar of midecamycin, and in transferring the mycaminose to the midecamycin aglycone in S. mycarofaciens. Similar to gene pairs desVIII/desVII in S. venezuelae and tylMIII/tylMII in S. fradiae, the product of midH probably functions as an auxiliary protein required by the MidI protein for efficient glycosyltransfer in midecamycin biosynthesis.
语种	英语
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/175437]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Institute of Chemical Microbiology, Bergische University 2.中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Cong Lina,Wolfgang PIEPERSBERG. cloningandcharacterizationofgenesencodedindtdpdmycaminosebiosyntheticpathwayfromamidecamycinproducingstrainstreptomycesmycarofaciens[J]. actabiochimicaetbiophysicasinica,2007,39(3):187.
APA	Cong Lina,&Wolfgang PIEPERSBERG.(2007).cloningandcharacterizationofgenesencodedindtdpdmycaminosebiosyntheticpathwayfromamidecamycinproducingstrainstreptomycesmycarofaciens.actabiochimicaetbiophysicasinica,39(3),187.
MLA	Cong Lina,et al."cloningandcharacterizationofgenesencodedindtdpdmycaminosebiosyntheticpathwayfromamidecamycinproducingstrainstreptomycesmycarofaciens".actabiochimicaetbiophysicasinica 39.3(2007):187.