Application of an in situ CO2-bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis

doi:10.1186/s13068-019-1523-7

	Application of an in situ CO2-bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis
	Qi, Man 2; Yao, Changhong 2; Sun, Binhuan 2; Cao, Xupeng 1,3,4; Fei, Qiang 5; Liang, Bobo 5; Ran, Wenyi 2; Xiang, Qi 2; Zhang, Yongkui 2; Lan, Xianqiu 2
刊名	BIOTECHNOLOGY FOR BIOFUELS
	2019-07-16
卷号	12 页码:21
关键词	Starch Nitrogen depletion pH Bicarbonate Amylose Tetraselmis subcordiformis
ISSN号	1754-6834
DOI	10.1186/s13068-019-1523-7
通讯作者	Yao, Changhong(yaochanghong@scu.edu.cn)
英文摘要	Background: Microalgal starch is regarded as a promising alternative to crop-based starch for biorefinery such as the production of biofuels and bio-based chemicals. The single or separate use of inorganic carbon source, e.g., CO2 and NaHCO3, caused aberrant pH, which restricts the biomass and starch production. The present study applied an in situ CO2-NaHCO3 system to regulate photosynthetic biomass and starch production along with starch quality in a marine green microalga Tetraselmis subcordiformis under nitrogen-depletion (-N) and nitrogen-limitation (+/- N) conditions. Results: The CO2 (2%)-NaHCO3 (1 g L-1) system stabilized the pH at 7.7 in the -N cultivation, under which the optimal biomass and starch accumulation were achieved. The biomass and starch productivity under -N were improved by 2.1-fold and 1.7-fold, respectively, with 1 g L-1 NaHCO3 addition compared with the one without NaHCO3 addition. NaHCO3 addition alleviated the high-dCO(2) inhibition caused by the single CO2 aeration, and provided sufficient effective carbon source HCO3- for the maintenance of adequate photosynthetic efficiency and increase in photoprotection to facilitate the biomass and starch production. The amylose content was also increased by 44% under this CO2-bicarbonate system compared to the single use of CO2. The highest starch productivity of 0.73g L(-1)day(-1) under -N cultivation and highest starch concentration of 4.14 g L-1 under +/- N cultivation were both achieved with the addition of 1 g L-1 NaHCO3. These levels were comparable to or exceeded the current achievements reported in studies. The addition of 5 g L-1 NaHCO3 under +/- N cultivation led to a production of high-amylose starch (59.3% of total starch), which could be used as a source of functional food. Conclusions: The in situ CO2-NaHCO3 system significantly improved the biomass and starch production in T. subcordiformis. It could also regulate the starch quality with varied relative amylose content under different cultivation modes for diverse downstream applications that could promote the economic feasibility of microalgal starch-based biofuel production. Adoption of this system in T. subcordiformis would facilitate the CO2 mitigation couple with its starch-based biorefinery.
资助项目	Fundamental Research Funds for the Central Universities[YJ201734] ; National Natural Science Foundation of China[41406177]
WOS关键词	DISSOLVED INORGANIC CARBON ; LIPID PRODUCTIVITY ; CELL-GROWTH ; CO2 ; BICARBONATE ; CULTIVATION ; PH ; ALGAE ; COST ; SUPPLEMENTATION
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	BMC
WOS记录号	WOS:000475760700001
资助机构	Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/173881]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Yao, Changhong
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Biotechnol Dept, Dalian 116023, Liaoning, Peoples R China 2.Sichuan Univ, Dept Pharmaceut & Biol Engn, Sch Chem Engn, Chengdu 610065, Sichuan, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Liaoning, Peoples R China 4.Dalian Natl Lab Clean Energy, Div Solar Energy, Dalian 116023, Liaoning, Peoples R China 5.Xi An Jiao Tong Univ, Sch Chem Engn & Technol, Xian 710049, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Qi, Man,Yao, Changhong,Sun, Binhuan,et al. Application of an in situ CO2-bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis[J]. BIOTECHNOLOGY FOR BIOFUELS,2019,12:21.
APA	Qi, Man.,Yao, Changhong.,Sun, Binhuan.,Cao, Xupeng.,Fei, Qiang.,...&Lan, Xianqiu.(2019).Application of an in situ CO2-bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis.BIOTECHNOLOGY FOR BIOFUELS,12,21.
MLA	Qi, Man,et al."Application of an in situ CO2-bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis".BIOTECHNOLOGY FOR BIOFUELS 12(2019):21.