Discovery of a New Biomarker Pattern for Differential Diagnosis of Acute Ischemic Stroke Using Targeted Metabolomics

doi:10.3389/fneur.2019.01011

	Discovery of a New Biomarker Pattern for Differential Diagnosis of Acute Ischemic Stroke Using Targeted Metabolomics
	Sun, Ruitan 2; Li, Yan 2; Cai, Ming 2; Cao, Yunfeng 1; Piao, Xiangyu 2
刊名	FRONTIERS IN NEUROLOGY
	2019-09-19
卷号	10 页码:7
关键词	metabolomics acute ischemic stroke biomarkers mass spectrometry amino acids carnitine
ISSN号	1664-2295
DOI	10.3389/fneur.2019.01011
通讯作者	Sun, Ruitan(sunruitan@126.com)
英文摘要	Stroke is one of the leading causes of disability all over the world. However, biomarkers for fast differential diagnosis of acute ischemic stroke (AIS) from vertigo or headache, remains lacking. Using a direct-infusion mass spectrometry method, it is possible to establish an efficient method for AIS differential diagnosis that requires only a few minutes. Thirty-eight clearly diagnosed AIS patients and 46 patients with a main complaint of vertigo were enrolled in this study. There was a total of 58 metabolites that were measured by our targeted metabolomics method, and the data were analyzed by pattern recognition algorithms. As a result, a clear classification between AIS and vertigo patients was achieved. Acylcarnitines are the major discriminating metabolites between the two groups. Arginine and its ratio, which is related to urea cycle metabolites, including arginine/ornithine and citrulline/arginine, also accounted for the classification. Interestingly, the levels of these metabolites were also found to be restored among recovering AIS patients (n = 11), which indicated that the metabolic alterations are possibly related to AIS development. Based on the characters from the data pattern reorganization, a novel biomarkers pattern was established using a binary logistic model, which contained arginine, arginine/ornithine, vaccenylcarnitine, and hydroxylbutyrylcarnitine. This biomarkers pattern achieved an area under the receiver operating characteristic curve of 0.89 for the differential diagnosis of AIS. Considering the efficiency and the diagnostic performance of the biomarkers pattern, our method has potential future use for the clinical application.
资助项目	Liaoning provincial education office[JYTFUDF201752]
WOS关键词	SERUM ; METABOLITES
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000486612200001
资助机构	Liaoning provincial education office ; Liaoning provincial education office ; Liaoning provincial education office ; Liaoning provincial education office ; Liaoning provincial education office ; Liaoning provincial education office ; Liaoning provincial education office ; Liaoning provincial education office
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/172758]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Sun, Ruitan
作者单位	1.Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian, Peoples R China 2.Dalian Univ, Dept Neurol, Affiliated Zhongshan Hosp, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Sun, Ruitan,Li, Yan,Cai, Ming,et al. Discovery of a New Biomarker Pattern for Differential Diagnosis of Acute Ischemic Stroke Using Targeted Metabolomics[J]. FRONTIERS IN NEUROLOGY,2019,10:7.
APA	Sun, Ruitan,Li, Yan,Cai, Ming,Cao, Yunfeng,&Piao, Xiangyu.(2019).Discovery of a New Biomarker Pattern for Differential Diagnosis of Acute Ischemic Stroke Using Targeted Metabolomics.FRONTIERS IN NEUROLOGY,10,7.
MLA	Sun, Ruitan,et al."Discovery of a New Biomarker Pattern for Differential Diagnosis of Acute Ischemic Stroke Using Targeted Metabolomics".FRONTIERS IN NEUROLOGY 10(2019):7.