Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori

doi:10.1016/j.jchromb.2019.04.039

	Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori
	Liu, Dian 1,2; Jin, Hongli 2; Wang, Jixia 2; Zhou, Han 2; Liu, Yanfang 2; Feng, Jiatao 2; Liang, Xinmiao 2
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2019-06-15
卷号	1118 页码:70-77
关键词	Caulis Polygoni Multiflori Polar compounds Polar-copolymerized reversed-phase chromatography Zwitterionic hydrophilic interaction chromatography
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2019.04.039
通讯作者	Liu, Yanfang(liuyanfang@dicp.ac.cn) ; Liang, Xinmiao(liangxm@dicp.ac.cn)
英文摘要	Polar compounds are abundant in the water decoction of Traditional Chinese Medicines (TCMs) and possess important biological activities. However, purifying these compounds has faced great difficulties largely due to poor retention and insufficient selectivity. To solve this problem, an offline orthogonal 2-D RPLC coupled with hydrophilic interaction chromatography (HILIC) method was developed to achieve purification of polar compounds from Caulis Polygoni Multiflori. A polar-copolymerized XAqua C18 column and a zwitterionic Click XIon column exhibited satisfactory retention and separation for polar compounds. Therefore, they were adopted to construct the offline 2-D LC system. Furthermore, the method presented a high orthogonality, which was calculated to be 72%. The)(Aqua C18 column was used in the first dimension to fractionate the 5.1 g polar fraction with a recovery of 85% within 10 h. In the second dimension, three representative fractions were purified using the Click XIon column. Finally, three compounds with purity higher than 95% were identified for the first time from this plant. This offline 2-D RPLC/HILIC method was shown to be an effective approach to purifying polar compounds from Caulis Polygoni Multiflori.
资助项目	State Key Program of National Natural Science of China[U1508221] ; National Science Foundation of China[31670374] ; National Science Foundation of China[81403100] ; Innovation Program of science and research from DICP, CAS[DICP TMSR201601] ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; BASIC COMPOUNDS ; MASS-SPECTROMETRY ; C18 COLUMN ; SEPARATION ; ALKALOIDS ; ONLINE ; COMPONENTS ; SYSTEM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000469153600010
资助机构	State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171945]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liu, Yanfang; Liang, Xinmiao
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dian,Jin, Hongli,Wang, Jixia,et al. Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1118:70-77.
APA	Liu, Dian.,Jin, Hongli.,Wang, Jixia.,Zhou, Han.,Liu, Yanfang.,...&Liang, Xinmiao.(2019).Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1118,70-77.
MLA	Liu, Dian,et al."Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1118(2019):70-77.