An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite | |
Sun SJ(孙树津)![]() ![]() ![]() ![]() ![]() ![]() | |
刊名 | REVIEW OF SCIENTIFIC INSTRUMENTS
![]() |
2019 | |
卷号 | 90期号:7页码:12 |
ISSN号 | 0034-6748 |
DOI | 10.1063/1.5087770 |
英文摘要 | Pathophysiological changes of astronauts under space microgravity involve complex factors and require an integrative perspective to fully understand the mechanisms. The readouts from space cell biology experiments strongly depend on the hardware and especially the cell bioreactor that is used in distinct spacecraft. Herein, a specialized cell culture bioreactor is designed for culturing mammalian cells on board the SJ-10 satellite. This hardware focuses mainly on satisfying the requirements of gas exchange, bubble separation, and flow control, as well as their functional and structural integration on cell culture within the technical and environmental constraints of the spacecraft platform under microgravity. A passive bubble separator is constructed and is connected in series to an individual cell culture chamber to remove the bubbles that were produced in orbit during cell growth. A moderate flow rate is preset to provide sufficient mass transfer and low shear stress in a well-designed flow circuit. Together with other modules of temperature control, in situ microscopic imaging, and online imaging acquisition, this novel space cell culture system is successfully used to culture human endothelial cells and rat bone marrow-derived mesenchymal stem cells in the SJ-10 mission. The advantages and shortcomings of the integration design are discussed for this type of the hardware. |
分类号 | Q3 |
资助项目 | National Natural Science Foundation of China[U1738115] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31627804] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[XDA04020202-17] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[XDA04020416] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[XDA04073800] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[QYZDJ-SSW-JSC018] ; National Key Research and Development Program of China[2016YFA0501601] |
WOS关键词 | MAMMALIAN-CELL ; BIOREACTOR ; MORPHOLOGY |
WOS研究方向 | Instruments & Instrumentation ; Physics |
语种 | 英语 |
WOS记录号 | WOS:000478661400017 |
资助机构 | National Natural Science Foundation of China ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences ; National Key Research and Development Program of China |
其他责任者 | Sun, Shujin ; Long, Mian |
内容类型 | 期刊论文 |
源URL | [http://dspace.imech.ac.cn/handle/311007/79494] ![]() |
专题 | 力学研究所_国家微重力实验室 |
推荐引用方式 GB/T 7714 | Sun SJ,Wang CZ,Bi YZ,et al. An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite[J]. REVIEW OF SCIENTIFIC INSTRUMENTS,2019,90(7):12. |
APA | 孙树津.,王成之.,毕于振.,李宁.,吕东媛.,...&龙勉.(2019).An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite.REVIEW OF SCIENTIFIC INSTRUMENTS,90(7),12. |
MLA | 孙树津,et al."An integration design of gas exchange, bubble separation, and flow control in a space cell culture system on board the SJ-10 satellite".REVIEW OF SCIENTIFIC INSTRUMENTS 90.7(2019):12. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论