Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis

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	Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis
	Jia XY(贾新颖) ; Tian ZH(田振华) ; Shao L(邵雷) ; Ju XD(瞿旭东) ; Zhao QF(赵群飞) ; Tang J(唐键) ; Tang GL(唐功利) ; Liu W(刘文)
刊名	Chem. Biol.
	2006
卷号	13 期号:6 页码:575-585
ISSN号	1074-5521
其他题名	遗传鉴定氯丝菌素生物合成基因簇作为螺4-羟基乙酰乙酸内酯类抗生素生物合成的模式
通讯作者	唐功利 ; 刘文
英文摘要	The biosynthetic gene cluster for chlorothricin (CHL) was localized to a 122 kb contiguous DNA from Streptomyces antibioticus DSM 40725, and its involvement in CHL biosynthesis was confirmed by gene inactivation and complementation. Bioinformatic analysis of the sequenced 111.989 kb DNA region revealed 42 open reading frames, 35 of which were defined to constitute the CHL gene cluster. An assembly model for CHL biosynthesis from D-olivose, 2-methoxy-5chloro-6-methylsalicyclic acid, and chlorothricolide building blocks was proposed. This work represents cloning of a gene cluster for spirotetronate antibiotic biosynthesis and sets the stage to investigate the unusual macrolide biosynthesis including tandem Diels-Alder cyclizations, Baeyer-Villiger oxidation, and incorporation of an enoylpyruvate unit.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.chembiol.2006.03.008
语种	英语
WOS记录号	WOS:000238723800006
公开日期	2013-08-14
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/27637]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	Jia XY,Tian ZH,Shao L,et al. Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis[J]. Chem. Biol.,2006,13(6):575-585.
APA	贾新颖.,田振华.,邵雷.,瞿旭东.,赵群飞.,...&刘文.(2006).Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis.Chem. Biol.,13(6),575-585.
MLA	贾新颖,et al."Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis".Chem. Biol. 13.6(2006):575-585.