Visualization of molecule interaction between antigen and antibody - One of ellipsometric imaging applications

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	Visualization of molecule interaction between antigen and antibody - One of ellipsometric imaging applications
	Jin G(靳刚); Meng YH(孟永宏); Xing JH(邢建华); Zhao ZY(赵子彦)
	1998
会议名称	Biomedical Optics and Lasers - Diagnostics and Treatment
会议日期	1998
会议地点	BEIJING, PEOPLES R CHINA, SEP 16-18, 1998
关键词	Engineering Biomedical Optics
中文摘要	It is to investigate molecule interactions between antigen and antibody with ellipsometric imaging technique and demonstrate some features and possibilities offered by applications of the technique. Molecule interaction is an important interest for molecule biologist and immunologist. They have used some established methods such as immufluorcence, radioimmunoassay and surface plasma resonance, etc, to study the molecule interaction. At the same time, experimentalists hope to use some updated technique with more direct visual results. Ellipsometric imaging is non-destructive and exhibits a high sensitivity to phase transitions with thin layers. It is capable of imaging local variations in the optical properties such as thickness due to the presence of different surface concentration of molecule or different deposited molecules. If a molecular mono-layer (such as antigen) with bio-activity were deposited on a surface to form a sensing surface and then incubated in a solution with other molecules (such as antibody), a variation of the layer thickness when the molecules on the sensing surface reacted with the others in the solution could be observed with ellipsometric imaging. Every point on the surface was measured at the same time with a high sensitivity to distinguish the variation between mono-layer and molecular complexes. Ellipsometric imaging is based on conventional ellipsometry with charge coupled device (CCD) as detector and images are caught with computer with image processing technique. It has advantages of high sensitivity to thickness variation (resolution in the order of angstrom), big field of view (in square centimeter), high sampling speed (a picture taken within one second), and high lateral resolution (in the order of micrometer). Here it has just shown one application in study of antigen-antibody interaction, and it is possible to observe molecule interaction process with an in-situ technique.
收录类别	CPCI-S
会议录	BIOMEDICAL OPTICS AND LASERS: DIAGNOSTICS AND TREATMENT(in Book Series: PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)),Volume: 3548 Pages: 131-135 Published: 1998
会议录出版者	SPIE - INT SOC OPTICAL ENGINEERING, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
学科主题	力学
语种	英语
ISSN号	0277-786X
ISBN号	0-8194-3009-9
WOS记录号	WOS:000076866800026
内容类型	会议论文
源URL	[http://dspace.imech.ac.cn/handle/311007/13859]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Jin G,Meng YH,Xing JH,et al. Visualization of molecule interaction between antigen and antibody - One of ellipsometric imaging applications[C]. 见:Biomedical Optics and Lasers - Diagnostics and Treatment. BEIJING, PEOPLES R CHINA, SEP 16-18, 1998. 1998.