Silencing Brassinosteroid Receptor BRI1 Impairs Herbivory-elicited Accumulation of Jasmonic Acid-isoleucine and Diterpene Glycosides, but not Jasmonic Acid and Trypsin Proteinase Inhibitors in Nicotiana attenuata

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	Silencing Brassinosteroid Receptor BRI1 Impairs Herbivory-elicited Accumulation of Jasmonic Acid-isoleucine and Diterpene Glycosides, but not Jasmonic Acid and Trypsin Proteinase Inhibitors in Nicotiana attenuata
	Yang, Da-Hai ; Baldwin, Ian T. ; Wu, Jianqiang
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2013
卷号	55 期号:6 页码:514-526
关键词	BRI1 herbivore insect jasmonic acid Nicotiana attenuata secondary metabolites SIGNAL-TRANSDUCTION INSECT HERBIVORES PLANT-RESPONSES ORAL SECRETIONS MANDUCA-SEXTA BIOSYNTHESIS DEFENSE RESISTANCE PERCEPTION KINASE
ISSN号	1672-9072
通讯作者	Wu, JQ (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China. ; jwu@ice.mpg.de
英文摘要	The brassinosteroid (BR) receptor, BR insensitive 1 (BRI1), plays a critical role in plant development, but whether BRI1-mediated BR signaling is involved in plant defense responses to herbivores was largely unknown. Here, we examined the function of BRI1 in the resistance of Nicotiana attenuata (Solanaceae) to its specialist insect herbivore Manduca sexta. Jasmonic acid (JA) and JA-isoleucine conjugate (JA-Ile) are important hormones that mediate resistance to herbivores and we found that after wounding or simulated herbivory NaBRI1 had little effect on JA levels, but was important for the induction of JA-Ile. Further experiments revealed that decreased JAR (the enzyme for JA-Ile production) activity and availability of Ile in NaBRI1-silenced plants were likely responsible for the low JA-Ile levels. Consistently, M. sexta larvae gained more weight on NaBRI1-silenced plants than on the control plants. Quantification of insect feeding-induced secondary metabolites revealed that silencing NaBRI1 resulted in decreased levels of carbon-rich defensive secondary metabolites (hydroxygeranyllinalool diterpene glycosides, chlorogenic acid, and rutin), but had little effect on the nitrogen-rich ones (nicotine and trypsin proteinase inhibitors). Thus, NaBRI1-mediated BR signaling is likely involved in plant defense responses to M. sexta, including maintaining JA-Ile levels and the accumulation of several carbon-rich defensive secondary metabolites.
学科主题	Biochemistry & Molecular Biology; Plant Sciences
收录类别	SCI
资助信息	Max Planck Society
语种	英语
公开日期	2013-07-24
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/16609]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
推荐引用方式 GB/T 7714	Yang, Da-Hai,Baldwin, Ian T.,Wu, Jianqiang. Silencing Brassinosteroid Receptor BRI1 Impairs Herbivory-elicited Accumulation of Jasmonic Acid-isoleucine and Diterpene Glycosides, but not Jasmonic Acid and Trypsin Proteinase Inhibitors in Nicotiana attenuata[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2013,55(6):514-526.
APA	Yang, Da-Hai,Baldwin, Ian T.,&Wu, Jianqiang.(2013).Silencing Brassinosteroid Receptor BRI1 Impairs Herbivory-elicited Accumulation of Jasmonic Acid-isoleucine and Diterpene Glycosides, but not Jasmonic Acid and Trypsin Proteinase Inhibitors in Nicotiana attenuata.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,55(6),514-526.
MLA	Yang, Da-Hai,et al."Silencing Brassinosteroid Receptor BRI1 Impairs Herbivory-elicited Accumulation of Jasmonic Acid-isoleucine and Diterpene Glycosides, but not Jasmonic Acid and Trypsin Proteinase Inhibitors in Nicotiana attenuata".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 55.6(2013):514-526.