Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro | |
Yang, Cuiqin ; Qin, Yaoguo ; Sun, Xin ; Yuan, Shu ; Lin, Honghui | |
刊名 | NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA |
2012 | |
卷号 | 40期号:1页码:107-112 |
关键词 | hyperhydricity leaf reversion tissue culture |
ISSN号 | 0255-965X |
通讯作者 | Yang, CQ (reprint author), Sichuan Univ, Key Lab Bioresources & Ecoenvironm, State Key Lab Hydraul & Mt River Engn, Minist Educ,Coll Life Sci, Chengdu 610065, Peoples R China. |
产权排序 | 3 |
英文摘要 | An efficient protocol was established for Sedum spectabile Boreau propagation. Various leaf parts were used as explants to regenerate planners, the stem segments of which were cultured for shoot proliferation and plantlet multiplication. The results showed that the leaf base was the optimal explant, as compared to both the middle and the top of leaves, for shoot formation. The highest shoot induction of 88.9% was observed on MS medium supplemented with 0.6 mg/l TDZ and 0.1 mg/l NAA. Hyperhydric leaves obtained in primary culture developed first into abnormal somatic embryos 10 days after subculture, and then into hyperhydric planners after an additional 10 days. The hyperhydric planners reversed to normal planners when plant growth regulators were removed from culture medium. Further, stem segments from reversed plantlets were used for shoot regeneration and root induction. Optimal shoot regeneration was obtained in MS medium containing 0.6 mg/l TDZ with 0.1 mg/l NAA. Root induction and root mean number were all higher on auxin-free medium than on medium containing auxins. |
学科主题 | Plant Sciences |
收录类别 | SCI |
资助信息 | National Nature Science Foundation of China [91017004, 31070210, 31000682, 30970214]; Sichuan Nature Science Foundation [2010JQ0080] |
语种 | 英语 |
WOS记录号 | WOS:000306538900015 |
公开日期 | 2013-07-03 |
内容类型 | 期刊论文 |
源URL | [http://210.75.237.14/handle/351003/23834] |
专题 | 成都生物研究所_生态研究 |
推荐引用方式 GB/T 7714 | Yang, Cuiqin,Qin, Yaoguo,Sun, Xin,et al. Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro[J]. NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA,2012,40(1):107-112. |
APA | Yang, Cuiqin,Qin, Yaoguo,Sun, Xin,Yuan, Shu,&Lin, Honghui.(2012).Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro.NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA,40(1),107-112. |
MLA | Yang, Cuiqin,et al."Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro".NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA 40.1(2012):107-112. |
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