H+ISFET-based biosensor for determination of penicillin G

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	H+ISFET-based biosensor for determination of penicillin G
	Liu JG ; Liang L ; Li GX ; Han RS ; Chen KM
刊名	biosensors & bioelectronics
	1998
卷号	13 期号:9 页码:1023-1028
关键词	H- ion sensitive field effect transistor (H+-ISFET) penicillin G acylase enzyme based FET biosensor (ENFET) determination of penicillin G ENZYME POTENTIOMETRIC SENSORS ELECTRODES ISFET FIELD-EFFECT TRANSISTOR
ISSN号	0956-5663
通讯作者	liu jg,acad sinica,inst microbiol,beijing 100080,peoples r china.
中文摘要	a biosensor based on an h+ ion sensitive field effect transistor (h+-isfet) and penicillin g acylase has been developed. the response time of the sensor to different concentrations of penicillin g was 30 s. in a 20 mm phosphate buffer at ph 7.0, the linear range of the calibration curve was from 0.5 to 8 mm. the coefficients of variation for three samples with 20 repeated measurements were below 5%. stability of the sensor could reach about 6 months and more than 1000 runs were performed without a significant decrease of the output value. the sensor was tested for measurement of the penicillin g content in penicillin fermentation broth. forty samples with low and high concentrations of penicillin g were chosen for the correlation test. the values assayed by the sensor method were compared with the values assayed by hplc method, the correlation coefficient (r) was 0.9944 and the regression equation was y = 1.034x - 2083.7 respectively. the different measuring methods are discussed in the text. (c) 1998 published by elsevier science s.a. all rights reserved.
学科主题	半导体器件
收录类别	SCI
语种	英语
公开日期	2010-08-12
内容类型	期刊论文
源URL	[http://ir.semi.ac.cn/handle/172111/13066]
专题	半导体研究所_中国科学院半导体研究所（2009年前）
推荐引用方式 GB/T 7714	Liu JG,Liang L,Li GX,et al. H+ISFET-based biosensor for determination of penicillin G[J]. biosensors & bioelectronics,1998,13(9):1023-1028.
APA	Liu JG,Liang L,Li GX,Han RS,&Chen KM.(1998).H+ISFET-based biosensor for determination of penicillin G.biosensors & bioelectronics,13(9),1023-1028.
MLA	Liu JG,et al."H+ISFET-based biosensor for determination of penicillin G".biosensors & bioelectronics 13.9(1998):1023-1028.