A Series of TA-Based and Zero-Background Vectors for Plant Functional Genomics | |
Wang, Chuntao1,2; Yin, Xianlun1,2; Kong, Xiangxiang1,3; Li, Wansha1,2; Ma, Lan1,3; Sun, Xudong1,2; Guan, Yanlong1,3; Todd, Christopher D.4; Yang, Yongping1,2![]() | |
刊名 | PLOS ONE
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2013-03-29 | |
卷号 | 8期号:3页码:e59576 |
关键词 | PROTEIN-KINASE SALT TOLERANCE ARABIDOPSIS VERSATILE CLONING EXPRESSION SYSTEM GENES TRANSFORMATION ENCODES |
ISSN号 | 1932-6203 |
通讯作者 | Hu, XY (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Yunna, Peoples R China. ; huxiangyang@mail.kib.ac.cn |
英文摘要 | With the sequencing of genomes from many organisms now complete and the development of high-throughput sequencing, life science research has entered the functional post-genome era. Therefore, deciphering the function of genes and how they interact is in greater demand. To study an unknown gene, the basic methods are either overexpression or gene knockout by creating transgenic plants, and gene construction is usually the first step. Although traditional cloning techniques using restriction enzymes or a site-specific recombination system (Gateway or Clontech cloning technology) are highly useful for efficiently transferring DNA fragments into destination plasmids, the process is time consuming and expensive. To facilitate the procedure of gene construction, we designed a TA-based cloning system in which only one step was needed to subclone a DNA fragment into vectors. Such a cloning system was developed from the pGreen binary vector, which has a minimal size and facilitates construction manipulation, combined with the negative selection marker gene ccdB, which has the advantages of eliminating the self-ligation background and directly enabling high-efficiency TA cloning technology. We previously developed a set of transient and stable transformation vectors for constitutive gene expression, gene silencing, protein tagging, subcellular localization analysis and promoter activity detection. Our results show that such a system is highly efficient and serves as a high-throughput platform for transient or stable transformation in plants for functional genome research. |
学科主题 | Science & Technology - Other Topics |
WOS标题词 | Science & Technology |
类目[WOS] | Multidisciplinary Sciences |
研究领域[WOS] | Science & Technology - Other Topics |
关键词[WOS] | PROTEIN-KINASE ; SALT TOLERANCE ; ARABIDOPSIS ; VERSATILE ; CLONING ; EXPRESSION ; SYSTEM ; GENES ; TRANSFORMATION ; ENCODES |
收录类别 | SCI |
资助信息 | National Sciences Foundation of China [30971452, 31170256]; Major Sciences and Technology Program [110201101003-TS-03]; Young Academic and Technical Leader Raising Foundation of Yunnan Province [2012HB041]; State Education Ministry |
语种 | 英语 |
WOS记录号 | WOS:000317263900018 |
公开日期 | 2013-06-08 |
内容类型 | 期刊论文 |
源URL | [http://ir.kib.ac.cn/handle/151853/16513] ![]() |
专题 | 昆明植物研究所_中国科学院青藏高原研究所昆明部 |
作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Yunna, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming, Yunna, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 0W0, Canada |
推荐引用方式 GB/T 7714 | Wang, Chuntao,Yin, Xianlun,Kong, Xiangxiang,et al. A Series of TA-Based and Zero-Background Vectors for Plant Functional Genomics[J]. PLOS ONE,2013,8(3):e59576. |
APA | Wang, Chuntao.,Yin, Xianlun.,Kong, Xiangxiang.,Li, Wansha.,Ma, Lan.,...&Hu, Xiangyang.(2013).A Series of TA-Based and Zero-Background Vectors for Plant Functional Genomics.PLOS ONE,8(3),e59576. |
MLA | Wang, Chuntao,et al."A Series of TA-Based and Zero-Background Vectors for Plant Functional Genomics".PLOS ONE 8.3(2013):e59576. |
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