Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein

	Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein
	Xue R ; Liu Y ; Zheng YZ ; Wu YJ ; Li XJ ; Pei FK ; Ni JZ
刊名	biopolymers
	2012
卷号	98 期号:1 页码:59-66
关键词	EMBRYOGENESIS-ABUNDANT PROTEINS TRANSGENIC RICE WATER-DEFICIT NMR DESICCATION GENE TOLERANCE STRESS HVA1 MOLECULES
ISSN号	0006-3525
通讯作者	pei fk
中文摘要	the occurrence of a highly conserved 11-mer repeating motif in the primary sequence is a major characteristic of group 3 late embryogenesis abundant (lea3) proteins, which are strongly associated with abiotic stress tolerance of the plants. in this study, the three-dimensional structure, mimetic membrane association, and salt effect for consensus 11-mer motif from soybean pm2 protein (lea3) were investigated in sodium dodecyl sulfate (sds) micelles by nmr techniques. it was shown that the 11-mer motif was disordered in aqueous solution, but adopted an alpha-helix in sds micelles. nmr diffusion measurements demonstrated that the 11-mer motif was associated with sds micelles. paramagnetic quenching nmr experiments further revealed the orientation of the 11-mer motif with respect to the mimetic membrane: the ordered n-terminal segment was inserted into the mimetic membrane, and the disordered c-terminal segment was exposed to water. in addition, salt addition could not change the secondary structure of the 11-mer motif, but might slightly alter the relative spatial position of some n-terminal residue atoms. these results implied that the 11-mer motif would take an important role in structural plasticity and membrane stabilization for lea3 proteins. (c) 2011 wiley periodicals, inc. biopolymers (pept sci) 98: 5966, 2012.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000311339000007
公开日期	2013-06-09
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48421]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Xue R,Liu Y,Zheng YZ,et al. Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein[J]. biopolymers,2012,98(1):59-66.
APA	Xue R.,Liu Y.,Zheng YZ.,Wu YJ.,Li XJ.,...&Ni JZ.(2012).Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein.biopolymers,98(1),59-66.
MLA	Xue R,et al."Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein".biopolymers 98.1(2012):59-66.