High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry Ligand Fishing Assay: A Method for Screening Triplex DNA Binders from Natural Plant Extracts

	High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry Ligand Fishing Assay: A Method for Screening Triplex DNA Binders from Natural Plant Extracts
	Xu NS ; Yang HM ; Cui M ; Wan CH ; Liu SY
刊名	analytical chemistry
	2012
卷号	84 期号:5 页码:2562-2568
关键词	CYCLOOXYGENASE-2 EXPRESSION CIRCULAR-DICHROISM ETHIDIUM-BROMIDE PROTEIN-PROTEIN NUCLEIC-ACIDS BINDING ALKALOIDS HELIX INTERCALATION SEQUENCE
ISSN号	0003-2700
通讯作者	liu sy
中文摘要	a novel ligand fishing assay was established to screen triplex dna binders from complicated samples by a combination of immobilization of triplex dna on agarose beads and high-performance liquid chromatography-electrospray ionization-mass spectrometry (hplc esi-ms). the biotinylated oligodeoxynucleotides were first bound to the streptavidin agarose beads and then incubated with the duplex dna as the baits for ligand fishing. this assay was validated by the testing ligand library consisting of coralyne, ethidium bromide, vitexin, and formononetin. the binding affinities of ligands to target dna were also obtained based on the calibration curves of ligands. two components (berberine and palmatine) in the extract of phellodendron chinense schneid cortexes were fished out as triplex dna binders by this assay, which indicated its feasibility for screening triplex dna binders from complicated samples. this preliminary assay can be used for not only screening binders of triplex dna from natural products extracts but also can obtain their binding affinity information.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000301021400065
公开日期	2013-06-08
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48274]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Xu NS,Yang HM,Cui M,et al. High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry Ligand Fishing Assay: A Method for Screening Triplex DNA Binders from Natural Plant Extracts[J]. analytical chemistry,2012,84(5):2562-2568.
APA	Xu NS,Yang HM,Cui M,Wan CH,&Liu SY.(2012).High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry Ligand Fishing Assay: A Method for Screening Triplex DNA Binders from Natural Plant Extracts.analytical chemistry,84(5),2562-2568.
MLA	Xu NS,et al."High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry Ligand Fishing Assay: A Method for Screening Triplex DNA Binders from Natural Plant Extracts".analytical chemistry 84.5(2012):2562-2568.