Haemodynamics-Driven Developmental Pruning of Brain Vasculature in Zebrafish
Du, JL
刊名PLOS BIOLOGY
2012
卷号10期号:8页码:-e1001374
关键词PROTEIN-COUPLED RECEPTOR IN-VIVO NEUROVASCULAR DEVELOPMENT MICROVASCULAR NETWORKS STRUCTURAL ADAPTATION ENDOTHELIAL-CELLS MOLECULAR-BASIS NERVOUS-SYSTEM SHEAR-STRESS BLOOD-FLOW
ISSN号1545-7885
通讯作者Chen, Q (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai, Peoples R China.,forestdu@ion.ac.cn
英文摘要The brain blood vasculature consists of a highly ramified vessel network that is tailored to meet its physiological functions. How the brain vasculature is formed has long been fascinating biologists. Here we report that the developing vasculature in the zebrafish midbrain undergoes not only angiogenesis but also extensive vessel pruning, which is driven by changes in blood flow. This pruning process shapes the initial exuberant interconnected meshwork into a simplified architecture. Using in vivo long-term serial confocal imaging of the same zebrafish larvae during 1.5-7.5 d post-fertilization, we found that the early formed midbrain vasculature consisted of many vessel loops and higher order segments. Vessel pruning occurred preferentially at loop-forming segments via a process mainly involving lateral migration of endothelial cells (ECs) from pruned to unpruned segments rather than EC apoptosis, leading to gradual reduction in the vasculature complexity with development. Compared to unpruned ones, pruned segments exhibited a low and variable blood flow, which further decreased irreversibly prior to the onset of pruning. Local blockade of blood flow with micro-bead obstruction led to vessel pruning, whereas increasing blood flow by noradrenergic elevation of heartbeat impeded the pruning process. Furthermore, the occurrence of vessel pruning could be largely predicted by haemodynamics-based numerical simulation of vasculature refinement. Thus, changes of blood flow drive vessel pruning via lateral migration of ECs, leading to the simplification of the vasculature and possibly efficient routing of blood flow in the developing brain.
学科主题Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
收录类别SCI
资助信息National Basic Research Program of China [2012CB945101, 2011CBA00400, 2006CB943800]; Shanghai government [06dj14010, 07pj14107]; Hundred Talents Program from Chinese Academy of Sciences
语种英语
公开日期2013-06-04
内容类型期刊论文
源URL[http://ir.sibs.ac.cn/handle/331001/2484]  
专题上海神经科学研究所_神经所(总)
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GB/T 7714
Du, JL. Haemodynamics-Driven Developmental Pruning of Brain Vasculature in Zebrafish[J]. PLOS BIOLOGY,2012,10(8):-e1001374.
APA Du, JL.(2012).Haemodynamics-Driven Developmental Pruning of Brain Vasculature in Zebrafish.PLOS BIOLOGY,10(8),-e1001374.
MLA Du, JL."Haemodynamics-Driven Developmental Pruning of Brain Vasculature in Zebrafish".PLOS BIOLOGY 10.8(2012):-e1001374.
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