Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds | |
S. A. Omar ; N. I. Elsheery ; H. M. Kalaji ; M. K. H. Ebrahim ; S. Pietkiewicz ; C. -H. Lee ; S. I. Allakhverdiev ; Zeng-Fu Xu | |
刊名 | Biochemistry (Moscow)
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2013 | |
卷号 | 78期号:5页码:485-495 |
中文摘要 | Plant dehydrin proteins (DHNs) are known to be important for environmental stress tolerance and are involved in various developmental processes. Two full-length cDNAs JcDHN-1 and JcDHN-2 encoding two dehydrins from Jatropha curcas seeds were identified and characterized. JcDHN-1 is 764 bp long and contains an open reading frame of 528 bp. The deduced JcDHN-1 protein has 175 a.a. residues that form a 19.3-kDa polypeptide with a predicted isoelectric point (pI) of 6.41. JcDHN-2 is 855 bp long and contains an open reading frame of 441 bp. The deduced JcDHN-2 protein has 156 a.a. residues that form a 17.1-kDa polypeptide with a predicted pI of 7.09. JcDHN-1 is classified as type Y3SK2 and JcDHN-2 is classified as type Y2SK2 according to the YSK shorthand for structural classification of dehydrins. Homology analysis indicates that both JcDHN-1 and JcDHN-2 share identity with DHNs of other plants. Analysis of the conserved domain revealed that JcDHN-2 has glycoside hydrolase GH20 super-family activity. Quantitative real time PCR analysis for JcDHN-1 and JcDHN-2 expression during seed development showed increasing gene expression of both their transcript levels along with the natural dehydration process during seed development. A sharp increase in JcDHN-2 transcript level occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. These results indicate that both JcDHNs have the potential to play a role in cell protection during dehydration occurring naturally during jatropha orthodox seed development. |
公开日期 | 2013-05-30 |
内容类型 | 期刊论文 |
源URL | [http://ir.xtbg.org.cn/handle/353005/4516] ![]() |
专题 | 西双版纳热带植物园_能源植物分子育种研究组 |
推荐引用方式 GB/T 7714 | S. A. Omar,N. I. Elsheery,H. M. Kalaji,et al. Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds[J]. Biochemistry (Moscow),2013,78(5):485-495. |
APA | S. A. Omar.,N. I. Elsheery.,H. M. Kalaji.,M. K. H. Ebrahim.,S. Pietkiewicz.,...&Zeng-Fu Xu.(2013).Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds.Biochemistry (Moscow),78(5),485-495. |
MLA | S. A. Omar,et al."Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds".Biochemistry (Moscow) 78.5(2013):485-495. |
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