Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells

CORC > 半导体研究所 > 中国科学院半导体研究所 > 中国科学院半导体研究所（2009年前）

	Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells
	Wu HJ (Wu Hui-Juan) ; Li XX (Li Xiao-Xin) ; Dong JQ (Dong Jian-Qiang) ; Pei WH (Pei Wei-Hua) ; Chen HD (Chen Hong-Da)
刊名	graefes archive for clinical and experimental ophthalmology
	2007
卷号	245 期号:1 页码:135-142
关键词	retinal pigment epithelium (RPE) subretinal microphotodiode array (MPDA) biocompatibility primary culture microporous filter PIGMENT EPITHELIAL-CELLS ANNEXIN-V EXPRESSION ARPE-19 LINE
ISSN号	0721-832x
通讯作者	li, xx, peking univ, dept ophthalmol, peoples hosp, beijing, peoples r china. e-mail: drlixiaoxin@vip.sina.com
中文摘要	background: subretinal microphotodiode array (mpda) is a type of visual prosthesis used for the implantation in the subretinal space of patients with progressive photoreceptor cell loss. the present study aimed to evaluate the effect of materials for mpda on the viability, apoptosis and barrier function of cultured pig retinal pigment epithelium (rpe) cells.methods: primary culture of pig rpe cells was performed and 24 pig eyes were used to start rpe culture. the third passage of the cultures was plated on different materials for mpda and mpdas. the tetrazolium dye-reduction assay (mtt) was used to determine rpe cell viability. flow cytometry was measured to indicate the apoptosis rates of rpe cells on different materials. rpe cells were also cultured on microporous filters, and the transepithelial resistance and permeability of the experimental molecule were measured to determine the barrier function.results: the data from all the methods indicated no significant difference between the materials groups and the control group, and the materials tested showed good biocompatibility.conclusions: the materials for mpda used in the present study had no direct toxicity to the rpe cells and did not release harmful soluble factors that affected the barrier function of rpe in vitro.
学科主题	光电子学
收录类别	SCI
语种	英语
公开日期	2010-04-11
内容类型	期刊论文
源URL	[http://ir.semi.ac.cn/handle/172111/10254]
专题	半导体研究所_中国科学院半导体研究所（2009年前）
推荐引用方式 GB/T 7714	Wu HJ ,Li XX ,Dong JQ ,et al. Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells[J]. graefes archive for clinical and experimental ophthalmology,2007,245(1):135-142.
APA	Wu HJ ,Li XX ,Dong JQ ,Pei WH ,&Chen HD .(2007).Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells.graefes archive for clinical and experimental ophthalmology,245(1),135-142.
MLA	Wu HJ ,et al."Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells".graefes archive for clinical and experimental ophthalmology 245.1(2007):135-142.