A label-free, G-quadruplex DNAzyme-based fluorescent probe for signal-amplified DNA detection and turn-on assay of endonuclease

	A label-free, G-quadruplex DNAzyme-based fluorescent probe for signal-amplified DNA detection and turn-on assay of endonuclease
	Zhou ZX ; Du Y ; Zhang LB ; Dong SJ
刊名	biosensors & bioelectronics
	2012
卷号	34 期号:1 页码:100-105
关键词	LINKED-IMMUNOSORBENT-ASSAY PEROXIDASE-ACTIVITY RESTRICTION ENDONUCLEASES MOLECULAR BEACONS CATALYTIC DNA CLEAVING DNA HYBRIDIZATION ENZYME CLEAVAGE COMPLEX
ISSN号	0956-5663
通讯作者	dong sj
中文摘要	a novel g-quadruplex dnazyme molecular beacon (g-dnazymemb) strategy is developed for assays of target dna and restriction endonuclease. the detection system consists of g-dnazymemb strand and a blocker dna by using the fluorescence of 2',7'-dichlorodihydrofluorescein diacetate (h(2)dcfda) catalyzed by g-dnazymemb as a signal reporter. g-dnazymemb exhibits peroxidase activity in its free hairpin structure, and forms a catalytically inactive hybrid when hybridized with blocker dna. upon displacement of blocker dna by target dna or cleavage by restriction endonuclease. g-dnazymemb is released and two lateral portions of g-dnazymemb form a g-quadruplex structure, resulting in the recovery of catalytic activity which acts as a cofactor to catalyze h2o2-mediated oxidation of h(2)dcfda. for dna detection system, exonuclease iii (exo iii)-catalyzed amplification strategy is introduced to improve the sensitivity and target dna could be detected as low as 0.1 pm. with respect to restriction endonuclease detection system, 0.1 u/ml ecori endonuclease could be detected and this method could be easily transported to other restriction endonuclease analysis by simply changing the recognition sequence. these results demonstrate that the proposed g-dnazymemb strategy could be used as a label-free, simple, sensitive and cost-effective approach in analysis of target dna and restriction endonuclease. (c) 2012 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000302886500015
公开日期	2013-05-22
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48223]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhou ZX,Du Y,Zhang LB,et al. A label-free, G-quadruplex DNAzyme-based fluorescent probe for signal-amplified DNA detection and turn-on assay of endonuclease[J]. biosensors & bioelectronics,2012,34(1):100-105.
APA	Zhou ZX,Du Y,Zhang LB,&Dong SJ.(2012).A label-free, G-quadruplex DNAzyme-based fluorescent probe for signal-amplified DNA detection and turn-on assay of endonuclease.biosensors & bioelectronics,34(1),100-105.
MLA	Zhou ZX,et al."A label-free, G-quadruplex DNAzyme-based fluorescent probe for signal-amplified DNA detection and turn-on assay of endonuclease".biosensors & bioelectronics 34.1(2012):100-105.