Direct Evidence of Lipid Rafts by in situ Atomic Force Microscopy
Cai MJ ; Zhao WD ; Shang X ; Jiang JG ; Ji HB ; Tang ZY ; Wang HD
刊名small
2012
卷号8期号:8页码:1243-1250
关键词DETERGENT-RESISTANT MEMBRANES CELL-MEMBRANES PROTEIN SURFACE MODEL CYCLODEXTRINS MICRODOMAINS DYNAMICS CAVEOLAE SKELETON
ISSN号1613-6810
通讯作者tang zy
中文摘要lipid rafts are membrane microdomains enriched with cholesterol, glycosphingolipids, and proteins. although they are broadly presumed to play a pivotal role in various cellular functions, there are still fierce debates about the composition, functions, and even existence of lipid rafts. here high-resolution and time-lapse in situ atomic force microscopy is used to directly confirm the existence of lipid rafts in native erythrocyte membranes. the results indicate some important aspects of lipid rafts: most of the lipid rafts are in the size range of 100300 nm and have irregular shape; the detergent-resistant membranes consist of cholesterol microdomains and are not likely the same as the lipid rafts; cholesterol contributes significantly to the formation and stability of the protein domains; and band iii is an important protein of lipid rafts in the inner leaflet of erythrocyte membranes, indicating that lipid rafts are exactly the functional domains in plasma membrane. this work provides direct evidence of the presence, size, and main constitutive protein of lipid rafts at a resolution of a few nanometers, which will pave the way for studying their structure and functions in detail.
收录类别SCI收录期刊论文
语种英语
WOS记录号WOS:000302800000016
公开日期2013-05-22
内容类型期刊论文
源URL[http://ir.ciac.jl.cn/handle/322003/48209]  
专题长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
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Cai MJ,Zhao WD,Shang X,et al. Direct Evidence of Lipid Rafts by in situ Atomic Force Microscopy[J]. small,2012,8(8):1243-1250.
APA Cai MJ.,Zhao WD.,Shang X.,Jiang JG.,Ji HB.,...&Wang HD.(2012).Direct Evidence of Lipid Rafts by in situ Atomic Force Microscopy.small,8(8),1243-1250.
MLA Cai MJ,et al."Direct Evidence of Lipid Rafts by in situ Atomic Force Microscopy".small 8.8(2012):1243-1250.
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