Biodegradable copolymers with identical cationic segments and their performance in siRNA delivery

	Biodegradable copolymers with identical cationic segments and their performance in siRNA delivery
	Qi RG ; Liu S ; Chen J ; Xiao HH ; Yan LS ; Huang YB ; Jing XB
刊名	journal of controlled release
	2012
卷号	159 期号:2 页码:251-260
关键词	GENE DELIVERY BLOCK-COPOLYMER MICELLES NANOPARTICLES POLYCATION COMPLEXES LIPOSOMES VECTOR CANCER PEG
ISSN号	0168-3659
通讯作者	jing xb
中文摘要	the biodegradable cationic copolymers monomethoxy poly(ethylene glycol)-block-poly(epsilon-caprolactone)-block-poly(l-lysine) (mpeg-b-pcl-b-pll, further abbr. as "m") with a pcl block of different lengths and with a triazole linkage between the pcl and pll blocks were synthesized via ring-opening polymerization of epsilon-caprolactone and l-lysine n-carboxyanhydride and click reactions. with the copolymer without the pcl block (mpeg-b-pll, further abbr. as "p") as a control, self-assembling and complexation of these copolymers with sirna were studied. sirna-loading capacity, sirna delivery performance and cellular uptake of the complexation products, namely complex from p (p-complex) and complexes from m1 and m2 (m1-complex and m2-complex), were further examined and correlated to their block-copolymer compositions. the results showed the block copolymers p, m1 and m2 have strong enough binding ability so that the complexes formed can resist the heparin displacement and rnase degradation. compared to p-complex, m-complexes have smaller size and higher particle density so that they can be internalized via endocytosis more easily than free sirna or p-complex and they display higher sirna delivery efficiency and higher gene silencing efficiency than p-complex. the silencing efficiency of micellar complex is close to that of lipofectamine (tm) 2000 and much better than pei-25 kda. therefore, mpeg-b-pcl-b-pll is expected to be a promising sirna carrier. (c) 2012 elsevier b.v. all rights reserved. gene delivery
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000305001100012
公开日期	2013-05-22
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48205]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Qi RG,Liu S,Chen J,et al. Biodegradable copolymers with identical cationic segments and their performance in siRNA delivery[J]. journal of controlled release,2012,159(2):251-260.
APA	Qi RG.,Liu S.,Chen J.,Xiao HH.,Yan LS.,...&Jing XB.(2012).Biodegradable copolymers with identical cationic segments and their performance in siRNA delivery.journal of controlled release,159(2),251-260.
MLA	Qi RG,et al."Biodegradable copolymers with identical cationic segments and their performance in siRNA delivery".journal of controlled release 159.2(2012):251-260.