A simple method for the analysis by MS/MS of underivatized amino acids on dry blood spots from newborn screening

	A simple method for the analysis by MS/MS of underivatized amino acids on dry blood spots from newborn screening
	Wang CY ; Zhang WY ; Song FR ; Liu ZQ ; Liu SY
刊名	amino acids
	2012
卷号	42 期号:5 页码:1889-1895
关键词	TANDEM MASS-SPECTROMETRY LIQUID-CHROMATOGRAPHIC ANALYSIS SYRUP-URINE-DISEASE DRIED-BLOOD INHERITED DISORDERS PHYSIOLOGICAL SAMPLES BIOLOGICAL INTEREST RAPID DIAGNOSIS INBORN-ERRORS BODY-FLUIDS
ISSN号	0939-4451
通讯作者	song fr
中文摘要	the analysis by electrospray-ionization tandem mass spectrometry of amino acids with butyl esterification and isotopically labeled internal standard is routine in newborn screening laboratories worldwide. in the present study, we established a direct analysis method of higher accuracy that uses a non-deuterated internal standard. the automatic sampler and the pump of an lc apparatus were used to inject sample and mobile phase to ms, but no lc column was needed. the dry blood spot (dbs) material was prepared at levels of low, medium and high concentration; the running time was 1 min. in parallel to the new procedure, we applied the established method to analyze nine amino acids on dbs of healthy newborns and phenylketonuria newborns. the newly proposed method of product ion confirmation scan along with multiple reaction monitoring resulted in a very accurate identification of each amino acid. our innovative protocol had high sensitivity and specificity in the analysis of cases of suspected metabolic diseases.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000302812700033
公开日期	2013-05-22
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48196]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang CY,Zhang WY,Song FR,et al. A simple method for the analysis by MS/MS of underivatized amino acids on dry blood spots from newborn screening[J]. amino acids,2012,42(5):1889-1895.
APA	Wang CY,Zhang WY,Song FR,Liu ZQ,&Liu SY.(2012).A simple method for the analysis by MS/MS of underivatized amino acids on dry blood spots from newborn screening.amino acids,42(5),1889-1895.
MLA	Wang CY,et al."A simple method for the analysis by MS/MS of underivatized amino acids on dry blood spots from newborn screening".amino acids 42.5(2012):1889-1895.