Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip

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	Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip
	Chau, LH; Liang WF(梁文峰); Cheung, FWK; Liu, WK; Li WJ(李文荣); Chen, SC; Lee, GB
刊名	PLOS ONE
	2013
卷号	8 期号:1 页码:1-8
关键词	ELECTRIC-FIELD DIELECTROPHORESIS MANIPULATION SEPARATION
ISSN号	1932-6203
通讯作者	Liu, WK ; 李文荣
产权排序	2
中文摘要	The use of optical dielectrophoresis (ODEP) to manipulate microparticles and biological cells has become increasingly popular due to its tremendous flexibility in providing reconfigurable electrode patterns and flow channels. ODEP enables the parallel and free manipulation of small particles on a photoconductive surface on which light is projected, thus eliminating the need for complex electrode design and fabrication processes. In this paper, we demonstrate that mouse cells comprising melan-a cells, RAW 267.4 macrophage cells, peripheral white blood cells and lymphocytes, can be manipulated in an opto-electrokinetics (OEK) device with appropriate DEP parameters. Our OEK device generates a non-rotating electric field and exerts a localized DEP force on optical electrodes. Hitherto, we are the first group to report that among all the cells investigated, melan-a cells, lymphocytes and white blood cells were found to undergo self-rotation in the device in the presence of a DEP force. The rotational speed of the cells depended on the voltage and frequency applied and the cells' distance from the optical center. We discuss a possible mechanism for explaining this new observation of induced self-rotation based on the physical properties of cells. We believe that this rotation phenomenon can be used to identify cell type and to elucidate the dielectric and physical properties of cells.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	ELECTRIC-FIELD ; DIELECTROPHORESIS ; MANIPULATION ; SEPARATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000313429800009
公开日期	2013-04-21
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/10636]
专题	沈阳自动化研究所_机器人学研究室
推荐引用方式 GB/T 7714	Chau, LH,Liang WF,Cheung, FWK,et al. Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip[J]. PLOS ONE,2013,8(1):1-8.
APA	Chau, LH.,Liang WF.,Cheung, FWK.,Liu, WK.,Li WJ.,...&Lee, GB.(2013).Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip.PLOS ONE,8(1),1-8.
MLA	Chau, LH,et al."Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip".PLOS ONE 8.1(2013):1-8.