In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand

	In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand
	Ai J ; Li T ; Li BL ; Xu YH ; Li D ; Liu ZJ ; Wang EK
刊名	analytica chimica acta
	2012
卷号	741 页码:93-99
关键词	RNA DNA NANOPARTICLES NUCLEOLIN BINDING SURFACE PROBE
ISSN号	0003-2670
通讯作者	wang ek
中文摘要	in this article, we reported a novel approach for in situ labeling and imaging hela cancer cells utilizing a bifunctional aptamer (as1411) and its fluorescent ligand, protoporphyrin ix (ppix). in the presence of potassium ion, as1411 folded to g-quadruplex structure, binded fluorescent ligand (ppix) with fluorescent enhancement, and targeted the nucleolin overexpressed by cancer cells. consequently, bioimaging of cancer cells specifically were realized by laser scanning confocal microscope. the bioimaging strategy with as1411-ppix complex was capable to distinguish hela cancer cells from normal cells unambiguously, and fluorescence imaging of cancer cells was also realized in human serum. moreover, the bioimaging method was very facile, effective and need not any covalent modification. these results illustrated that the useful approach can provide a novel clue for bioimaging based on non-covalent bifunctional aptamer in clinic diagnosis. (c) 2012 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000307689800012
公开日期	2013-04-23
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/47952]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Ai J,Li T,Li BL,et al. In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand[J]. analytica chimica acta,2012,741:93-99.
APA	Ai J.,Li T.,Li BL.,Xu YH.,Li D.,...&Wang EK.(2012).In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand.analytica chimica acta,741,93-99.
MLA	Ai J,et al."In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand".analytica chimica acta 741(2012):93-99.