Expression and characterization of a new valosin-containing protein from silkworm

	Expression and characterization of a new valosin-containing protein from silkworm
	Chen P ; Wang RR ; Peng Z ; Liu Q ; Ni JZ
刊名	insect science
	2012
卷号	19 期号:5 页码:549-558
关键词	BOMBYX-MORI DOMESTICATED SILKWORM ELEVATED EXPRESSION SEQUENCE ALIGNMENT SWISS-MODEL VCP DEGRADATION PROGNOSIS CARCINOMA INVOLVEMENT
ISSN号	1672-9609
通讯作者	ni jz
中文摘要	valosin-containing protein (vcp) is a type-ii adenosine triphosphatase (atpase) wih extensive biological function in organisms. silkworm is the second insect model for genetic studies and a bioreactor for proteinaceous drugs and biomaterials. in this paper, a new vcp-like gene was amplified from the fat body of silkworm following genome prediction and spliced expressed sequence tag sequences, using both reverse transcription polymerase chain reaction (rt-pcr) and 3'-race (rapid amplification of complementary dna ends) methods. bioinformatical analysis showed that the translated amino acid sequence contained a highly conserved domain of vcps similar to that of many insects. this domain consists of the conserved structure motifs of the atp binding site and the catalytical center, which is closely related to the insect vcps in a phylogenetic tree. the silkworm vcp-like gene was successfully inserted into the plasmid and transformed into escherichia coli cells to express vcp-like protein with atpase activity. the expression of silkworm vcp-like protein was also confirmed by western blotting and mass spectrometric analyses. distribution of the vcp-like gene in various tissues of the silkworm was also studied by real-time pcr. results showed that the messenger rna (mrna) of vcp-like protein is widely expressed in fat body, reproductive organs (testis or ovary), silk gland, head, malpighian tubule, epidermis and midgut. among them, fat body has the highest mrna expression level of the vcp-like gene, while the midgut has the lowest expression level. this study provides groundwork for further study on the structure and function of the new vcp-like protein.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000308505000002
公开日期	2013-04-23
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/47931]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Chen P,Wang RR,Peng Z,et al. Expression and characterization of a new valosin-containing protein from silkworm[J]. insect science,2012,19(5):549-558.
APA	Chen P,Wang RR,Peng Z,Liu Q,&Ni JZ.(2012).Expression and characterization of a new valosin-containing protein from silkworm.insect science,19(5),549-558.
MLA	Chen P,et al."Expression and characterization of a new valosin-containing protein from silkworm".insect science 19.5(2012):549-558.