Lighting-Up of the Dye Malachite Green with Mercury(II)-DNA and Its Application for Fluorescence Turn-Off Detection of Cysteine and Glutathione

	Lighting-Up of the Dye Malachite Green with Mercury(II)-DNA and Its Application for Fluorescence Turn-Off Detection of Cysteine and Glutathione
	Jia XF ; Li J ; Wang EK
刊名	chemistry-a european journal
	2012
卷号	18 期号:42 页码:13494-13500
关键词	HOST-GUEST COMPLEXATION TRIPHENYLMETHANE DYES TRIARYLMETHANE DYES AQUEOUS-SOLUTION G-QUADRUPLEX BASE-PAIRS DNA BINDING NEUROTOXICITY APTAMER
ISSN号	0947-6539
通讯作者	wang ek
中文摘要	this work describes a novel strategy for the highly sensitive and selective detection of cysteine (cys) and glutathione (gsh) based on the hg2+agro100malachite green (mg) complex system. the dye mg, which has a very low quantum yield in aqueous solution by itself, can bind with the thymine-rich dna agro100 in the presence of hg2+ ions to generate a striking fluorescence intensity enhancement of 1000-fold. as sulfur-containing amino acids, cys and gsh effectively sequester hg2+ ions from the hg2+agro100mg complex structure to switch the lit-up chemosensor to the off state (about a 50-fold fluorescence intensity decrease), thus providing a facile, but effective, method to probe for cys/gsh. the fluorescence titration, uv absorption, cd, and raman spectra provide some insight into the structural and chemical basis for the enhancement effect. the formation of the hg2+agro100mg complex significantly affects the electronic structure and conformation of the mg molecule by leading to an extended p system, which is the likely origin of the observed striking fluorescence intensity enhancement. notably, the proposed sensing platform exhibits exquisite selectivity and sensitivity toward cys/gsh with limits of detection of 5 nm for cys and 10 nm for gsh, respectively. furthermore, the straightforward assay design avoids labeling of the probe, uses only commercially available materials, and still displays comparable sensitivity and excellent selectivity.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000309597000035
公开日期	2013-04-23
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/47915]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Jia XF,Li J,Wang EK. Lighting-Up of the Dye Malachite Green with Mercury(II)-DNA and Its Application for Fluorescence Turn-Off Detection of Cysteine and Glutathione[J]. chemistry-a european journal,2012,18(42):13494-13500.
APA	Jia XF,Li J,&Wang EK.(2012).Lighting-Up of the Dye Malachite Green with Mercury(II)-DNA and Its Application for Fluorescence Turn-Off Detection of Cysteine and Glutathione.chemistry-a european journal,18(42),13494-13500.
MLA	Jia XF,et al."Lighting-Up of the Dye Malachite Green with Mercury(II)-DNA and Its Application for Fluorescence Turn-Off Detection of Cysteine and Glutathione".chemistry-a european journal 18.42(2012):13494-13500.