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	Protein A for human IgG oriented immobilization on silicon surface for an imaging ellipsometry biosensor (EI CONFERENCE)
	Meng Y. ; Chen B.
	2009
会议名称	31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, September 2, 2009 - September 6, 2009
会议地点	Minneapolis, MN, United states
关键词	Imaging ellipsometry biosensor was developed on the basement of imaging ellipsometry for thin-film and surface characterization. Using protein A to immobilize Human IgG on silicon surface for the imaging ellipsometry biosensor immunoassay was studied. As a result The Human IgG immobilized on silicon surface by protein A bound much more polyclonal antibody than that on hydrophobic surface directly which indicated that protein A could be used to immobilize Human IgG molecules in a highly oriented manner and Human IgG molecule could maintain its native configuration well on the silicon surface. Furthermore the absorption quantity of Protein A affecting on interaction of Human IgG and antibody molecules was investigated. The surface concentration of Human IgG and antibody molecules was enhanced as well as the increasing of the absorption quantity of Protein A but there are no obvious increase when the ratio of surface concentration of Protein A layer to that of saturated adsorption is larger than 80%. However even decrease slightly. 2009 IEEE.
页码	1497-1500
收录类别	EI
内容类型	会议论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/33035]
专题	长春光学精密机械与物理研究所_中科院长春光机所知识产出_会议论文
推荐引用方式 GB/T 7714	Meng Y.,Chen B.. Protein A for human IgG oriented immobilization on silicon surface for an imaging ellipsometry biosensor (EI CONFERENCE)[C]. 见:31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, September 2, 2009 - September 6, 2009. Minneapolis, MN, United states.

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