Novel HMW glutenin genes from Aegilops tauschii and their unique structures

	Novel HMW glutenin genes from Aegilops tauschii and their unique structures
	Chen, Wen-Jie 1; Yuan, Zhong-Wei 1; Zhang, Lian-Quan 1; Fan, Xing 1; Yan, Ze-Hong 1; Wang, Ji-Rui 1; Zheng, You-Liang 1; Zhang, Huai-Gang 2; Liu, Deng-Cai 1,2
刊名	genes & genomics
	2012-06-01
卷号	34 期号:3 页码:339-343
关键词	Aegilops tauschii Bacterial expression HMW-GS Phylogenetic analysis
ISSN号	1976-9571
中文摘要	a pair of novel high-molecular-weight glutenin subunits (hmw-gs) 1dx5.3(t) and 1dy12.1(t) were revealed and characterized from ae. tauschii accession pi554324. sds-page band of 1dx5.3(t) was between those of 1bx6 and 1bx7, while 1dy12.1(t) with slightly faster migration rate than that of 1dy12. the lengths of 1dx5.3 (t) and 1dy12.1 (t) were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues, respectively. their authenticity was confirmed by successful expression of the coding regions in escherichia coli. 1dx5.3(t) is the shortest of the known dx-type alleles. 1dy12.1(t) is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. this cysteine that is not existed in previously reported dy-type genes may be useful for improving bread wheat quality. median-joining network analysis indicated that 1dy12.1** (t) may be a key site in the genealogy of the glu-dy.
英文摘要	a pair of novel high-molecular-weight glutenin subunits (hmw-gs) 1dx5.3(t) and 1dy12.1(t) were revealed and characterized from ae. tauschii accession pi554324. sds-page band of 1dx5.3(t) was between those of 1bx6 and 1bx7, while 1dy12.1(t) with slightly faster migration rate than that of 1dy12. the lengths of 1dx5.3 (t) and 1dy12.1 (t) were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues, respectively. their authenticity was confirmed by successful expression of the coding regions in escherichia coli. 1dx5.3(t) is the shortest of the known dx-type alleles. 1dy12.1(t) is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. this cysteine that is not existed in previously reported dy-type genes may be useful for improving bread wheat quality. median-joining network analysis indicated that 1dy12.1** (t) may be a key site in the genealogy of the glu-dy.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; biotechnology & applied microbiology ; genetics & heredity
研究领域[WOS]	biochemistry & molecular biology ; biotechnology & applied microbiology ; genetics & heredity
关键词[WOS]	molecular-weight subunits ; bread-making quality ; d-genome ; triticum-tauschii ; allelic variation ; hexaploid wheat ; identification ; expression ; proteins
收录类别	SCI
语种	英语
WOS记录号	WOS:000305695300012
公开日期	2013-03-15
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/3660]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Sichuan, Peoples R China 2.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Chen, Wen-Jie,Yuan, Zhong-Wei,Zhang, Lian-Quan,et al. Novel HMW glutenin genes from Aegilops tauschii and their unique structures[J]. genes & genomics,2012,34(3):339-343.
APA	Chen, Wen-Jie.,Yuan, Zhong-Wei.,Zhang, Lian-Quan.,Fan, Xing.,Yan, Ze-Hong.,...&Liu, Deng-Cai.(2012).Novel HMW glutenin genes from Aegilops tauschii and their unique structures.genes & genomics,34(3),339-343.
MLA	Chen, Wen-Jie,et al."Novel HMW glutenin genes from Aegilops tauschii and their unique structures".genes & genomics 34.3(2012):339-343.