Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio
	Nie, P ; Hoole, D
刊名	JOURNAL OF HELMINTHOLOGY
	2000-09-01
卷号	74 期号:3 页码:253-257
关键词	LEUKOCYTE MIGRATION FISH LEUKOCYTES CHEMOATTRACTANTS HOST
ISSN号	0022-149X
通讯作者	Hoole, D, Univ Keele, Sch Life Sci, Parasitol Res Lab, Ctr Appl Entomol & Parasitol, Huxley Bldg, Keele ST5 5BG, Staffs, England
中文摘要	An in vitro assay was used to examine the effect of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda: Pseudophyllidea) on the polarization response of pronephric leucocytes of carp, Cyprinus carpio. Leucocytes, isolated from naive, naturally-infected fish and carp injected intraperitoneally with cestode extracts, were exposed to parasite extracts (protein concentrations 0-10.0 mu g ml(-1)), for up to 24 h in the presence or absence of carp serum. In general, polarization responses of the pronephric leucocytes, primarily neutrophils and eosinophils, increased with incubation time although there was no significant difference in the response induced by the different protein concentrations. Differences in the polarization response were, however, observed in naive, naturally infected and injected fish and the cells responded differently in the presence and absence of carp serum. In the absence of carp serum the polarization response of pronephric leucocytes in vitro was significantly reduced with cells obtained from injected and naturally infected fish compared with those obtained from naive carp. This suppression of leucocyte migration was however reduced by the addition of carp serum to the in vitro system. The role of this interaction between the possible suppression of polarization induced by the parasite and stimulation by serum is discussed.
英文摘要	An in vitro assay was used to examine the effect of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda: Pseudophyllidea) on the polarization response of pronephric leucocytes of carp, Cyprinus carpio. Leucocytes, isolated from naive, naturally-infected fish and carp injected intraperitoneally with cestode extracts, were exposed to parasite extracts (protein concentrations 0-10.0 mu g ml(-1)), for up to 24 h in the presence or absence of carp serum. In general, polarization responses of the pronephric leucocytes, primarily neutrophils and eosinophils, increased with incubation time although there was no significant difference in the response induced by the different protein concentrations. Differences in the polarization response were, however, observed in naive, naturally infected and injected fish and the cells responded differently in the presence and absence of carp serum. In the absence of carp serum the polarization response of pronephric leucocytes in vitro was significantly reduced with cells obtained from injected and naturally infected fish compared with those obtained from naive carp. This suppression of leucocyte migration was however reduced by the addition of carp serum to the in vitro system. The role of this interaction between the possible suppression of polarization induced by the parasite and stimulation by serum is discussed.
学科主题	Parasitology; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Parasitology ; Zoology
研究领域[WOS]	Parasitology ; Zoology
关键词[WOS]	LEUKOCYTE MIGRATION ; FISH LEUKOCYTES ; CHEMOATTRACTANTS ; HOST
收录类别	SCI
语种	英语
WOS记录号	WOS:000088860800010
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/10100]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Univ Keele, Sch Life Sci, Parasitol Res Lab, Ctr Appl Entomol & Parasitol, Keele ST5 5BG, Staffs, England 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Nie, P,Hoole, D. Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio[J]. JOURNAL OF HELMINTHOLOGY,2000,74(3):253-257.
APA	Nie, P,&Hoole, D.(2000).Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio.JOURNAL OF HELMINTHOLOGY,74(3),253-257.
MLA	Nie, P,et al."Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio".JOURNAL OF HELMINTHOLOGY 74.3(2000):253-257.