Potentiometric determination of trypsin using a polymeric membrane polycation-sensitive electrode based on current-controlled reagent delivery

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Potentiometric determination of trypsin using a polymeric membrane polycation-sensitive electrode based on current-controlled reagent delivery
	Chen, Yan 1,2; Ding, Jiawang1 ; Qin, Wei1
刊名	BIOELECTROCHEMISTRY
	2012-12-01
卷号	88 页码:144-147
关键词	Potentiometry Polycation-sensitive electrode Controlled release Protease Trypsin
ISSN号	1567-5394
通讯作者	Qin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, YICCAS, Key Lab Coastal Zone Environm Proc,Yantai Inst Co, Yantai 264003, Shandong, Peoples R China.
产权排序	[Chen, Yan; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, YICCAS, Key Lab Coastal Zone Environm Proc,Yantai Inst Co, Yantai 264003, Shandong, Peoples R China; [Chen, Yan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
中文摘要	A potentiometric biosensor for the determination of trypsin is described based on current-controlled reagent delivery. A polymeric membrane protamine-sensitive electrode with dinonylnaphthalene sulfonate as cation exchanger is used for in situ generation of protamine. Diffusion of protamine across the polymeric membrane can be controlled precisely by applying an external current. The hydrolysis catalyzed with trypsin in sample solution decreases the concentration of free protamine released at the sample-membrane interface and facilitates the stripping of protamine out of the membrane surface via the ion-exchange process with sodium ions from the sample solution, thus decreasing the membrane potential, by which the protease can be sensed potentiometrically. The influences of anodic current amplitude, current pulse duration and protamine concentration in the inner filling solution on the membrane potential response have been studied. Under optimum conditions, the proposed protamine-sensitive electrode is useful for continuous and reversible detection of trypsin over the concentration range of 0.5-5 U mL(-1) with a detection limit of 0.3 U mL(-1). The proposed detection strategy provides a rapid and reagentless way for the detection of protease activities and offers great potential in the homogeneous immunoassays using proteases as labels. (C) 2012 Elsevier B.V. All rights reserved.
英文摘要	A potentiometric biosensor for the determination of trypsin is described based on current-controlled reagent delivery. A polymeric membrane protamine-sensitive electrode with dinonylnaphthalene sulfonate as cation exchanger is used for in situ generation of protamine. Diffusion of protamine across the polymeric membrane can be controlled precisely by applying an external current. The hydrolysis catalyzed with trypsin in sample solution decreases the concentration of free protamine released at the sample-membrane interface and facilitates the stripping of protamine out of the membrane surface via the ion-exchange process with sodium ions from the sample solution, thus decreasing the membrane potential, by which the protease can be sensed potentiometrically. The influences of anodic current amplitude, current pulse duration and protamine concentration in the inner filling solution on the membrane potential response have been studied. Under optimum conditions, the proposed protamine-sensitive electrode is useful for continuous and reversible detection of trypsin over the concentration range of 0.5-5 U mL(-1) with a detection limit of 0.3 U mL(-1). The proposed detection strategy provides a rapid and reagentless way for the detection of protease activities and offers great potential in the homogeneous immunoassays using proteases as labels. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Biophysics; Electrochemistry
研究领域[WOS]	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Biophysics ; Electrochemistry
关键词[WOS]	BIOANALYTICAL APPLICATIONS ; ELECTROCHEMICAL ASSAY ; PROTEASE ACTIVITY ; POLYION SENSORS ; SUBSTRATE ; INHIBITORS ; BLOOD
收录类别	SCI
资助信息	National Natural Science Foundation of China [41176081]; Outstanding Youth Natural Science Foundation of Shandong Province [JQ200814]; Taishan Scholar Program of Shandong Province [TS20081159]
原文出处	http://dx.doi.org/j.bioelechem.2012.04.002
语种	英语
WOS记录号	WOS:000311065000022
公开日期	2013-03-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6037]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, YICCAS, Key Lab Coastal Zone Environm Proc,Yantai Inst Co, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yan,Ding, Jiawang,Qin, Wei. Potentiometric determination of trypsin using a polymeric membrane polycation-sensitive electrode based on current-controlled reagent delivery[J]. BIOELECTROCHEMISTRY,2012,88:144-147.
APA	Chen, Yan,Ding, Jiawang,&Qin, Wei.(2012).Potentiometric determination of trypsin using a polymeric membrane polycation-sensitive electrode based on current-controlled reagent delivery.BIOELECTROCHEMISTRY,88,144-147.
MLA	Chen, Yan,et al."Potentiometric determination of trypsin using a polymeric membrane polycation-sensitive electrode based on current-controlled reagent delivery".BIOELECTROCHEMISTRY 88(2012):144-147.