Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure
	Zhang Linbao 1,2; Wu Huifeng1 ; Liu Xiaoli 1,2; Chen Leilei 1; Wang Qing1 ; Zhao Jianmin1 ; You Liping 1,2
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2012-05-01
卷号	30 期号:3 页码:413-423
关键词	Venerupis philippinarum Glutathione S-transferase mRNA expression heavy metals benzo[a]pyrene biomarker
ISSN号	0254-4059
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China.,hfwu@yic.ac.cn ; jmzhao@yic.ac.cn
产权排序	[Zhang Linbao; Wu Huifeng; Liu Xiaoli; Chen Leilei; Wang Qing; Zhao Jianmin; You Liping] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang Linbao; Liu Xiaoli; You Liping] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
中文摘要	Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. We identified two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profiles of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these findings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.
英文摘要	Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. We identified two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profiles of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these findings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.
学科主题	Limnology; Oceanography
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
关键词[WOS]	OYSTER CRASSOSTREA-GIGAS ; HALIOTIS-DISCUS-DISCUS ; MESSENGER-RNA ; MYTILUS-GALLOPROVINCIALIS ; AROMATIC-HYDROCARBONS ; TIGRIOPUS-JAPONICUS ; DNA-DAMAGE ; MU-CLASS ; COPPER ; BIOMARKERS
收录类别	SCI
资助信息	Chinese Academy of Sciences; Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology[201115]; State Oceanic Administration of China
语种	英语
WOS记录号	WOS:000303680400008
公开日期	2013-03-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6015]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室海岸带生物资源高效利用研究与发展中心
作者单位	1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang Linbao,Wu Huifeng,Liu Xiaoli,et al. Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(3):413-423.
APA	Zhang Linbao.,Wu Huifeng.,Liu Xiaoli.,Chen Leilei.,Wang Qing.,...&You Liping.(2012).Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(3),413-423.
MLA	Zhang Linbao,et al."Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.3(2012):413-423.