Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion

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	Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion
	Hongtuo, F ; Chingjiang, W
刊名	AQUACULTURE RESEARCH
	2001-04-01
卷号	32 期号:4 页码:267-275
关键词	electrofusion nuclear transfer UV inactivation fish oocyte blastomere gastrula cell
ISSN号	1355-557X
通讯作者	Hongtuo, F, Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China
中文摘要	To study nuclear transfer in the leach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 degreesC in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days, It was found that gastrula cells incubated at 4 degreesC had the same totipotence as blastula cells, The optimal UV dosage for inactivation of the oocyte chromatin was 180-240 mJ cm(-2). Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development.
英文摘要	To study nuclear transfer in the leach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 degreesC in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days, It was found that gastrula cells incubated at 4 degreesC had the same totipotence as blastula cells, The optimal UV dosage for inactivation of the oocyte chromatin was 180-240 mJ cm(-2). Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development.
学科主题	Fisheries
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries
研究领域[WOS]	Fisheries
关键词[WOS]	CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; ELECTRIC-FIELD ; MOUSE EMBRYO ; TRANSPLANTATION ; INDUCTION ; FUSION ; SURVIVAL ; LINE
收录类别	SCI
语种	英语
WOS记录号	WOS:000168005600003
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/10022]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Hongtuo, F,Chingjiang, W. Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion[J]. AQUACULTURE RESEARCH,2001,32(4):267-275.
APA	Hongtuo, F,&Chingjiang, W.(2001).Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion.AQUACULTURE RESEARCH,32(4),267-275.
MLA	Hongtuo, F,et al."Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion".AQUACULTURE RESEARCH 32.4(2001):267-275.