The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase

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	The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase
	Li J(李洁) ; Gan JH(甘建华) ; 斯科特, 马修斯 ; Xia ZX(夏宗芗)
刊名	Biochem. Biophys. Res. Commun.
	2011
卷号	406 期号:4 页码:621-626
ISSN号	0006-291X
其他题名	酶反应诱导的甲醇脱氢酶中辅基PPQ(吡咯喹啉醌)的构象变化
通讯作者	夏宗芗
英文摘要	Methanol dehydrogenase is a heterotetrameric enzyme containing the prosthetic group pyrroloquinoline quinone (PQQ), which catalyzes the oxidation of methanol to formaldehyde. The crystal structure of methanol dehydrogenase from Methylophilus W3A1, previously determined at high resolution, exhibits a non-planar configuration of the PQQ ring system and lends support for a hydride transfer mechanism of the enzymatic reaction catalyzed by the enzyme. To investigate why PQQ is in the C5-reduced form and to better understand the catalytic mechanism of the enzyme, three structures of this enzyme in a new crystal form have been determined at higher resolution. Two of the three crystals were grown in the presence of 1 and 50 mM methanol, respectively, both structures of which show non-planar configurations of the PQQ ring system, confirming the previous conclusion: the other was crystallized in the presence of 50 mM ethanol, the structure of which displays a planar ring system for PQQ. Comparison of these structures reveals that the configuration change of PQQ is induced by the enzymatic reaction. The reaction takes place and the C5-reduced PQQ intermediate is produced when the enzyme co-crystallizes with methanol, but the enzymatic reaction does not take place and the PQQ ring retains a planar configuration of the oxidized orthoquinone form when ethanol instead of methanol is present in the crystallization solution. (C) 2011 Elsevier Inc. All rights reserved.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.bbrc.2011.02.107
语种	英语
WOS记录号	WOS:000289036600023
公开日期	2013-03-11
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/23700]
专题	上海有机化学研究所_生命有机化学国家重点实验室
推荐引用方式 GB/T 7714	Li J,Gan JH,斯科特, 马修斯,et al. The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase[J]. Biochem. Biophys. Res. Commun.,2011,406(4):621-626.
APA	李洁,甘建华,斯科特, 马修斯,&夏宗芗.(2011).The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase.Biochem. Biophys. Res. Commun.,406(4),621-626.
MLA	李洁,et al."The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase".Biochem. Biophys. Res. Commun. 406.4(2011):621-626.