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	Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator
	Gan, NQ; Cai, RX; Lin, ZX; Song, LR
刊名	ANALYST
	2003
卷号	128期号:3页码:301-305
关键词	MECHANISM
ISSN号	0003-2654
通讯作者	Cai, RX, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	This paper reports a new method for detection of ROS scavengers including superoxide dismutase, ascorbic acid and glutathione based on a 'probe' of peroxidase-oxidase biochemical oscillator. The oscillation period and amplitude change with different concentrations of scavengers. The linear ranges of superoxide dismutase, ascorbic acid and glutathione are respectively 1.56 x 10(-4)-1.56 x 10(-3) mg mL(-1), 1.75 x 10(-7) -1.75 x 10(-5) mol L-1 and 9.38 x 10(-7) -7.5 x 10(-5) mol L-1. The selectivity, linearity and precision for superoxide dismutase, ascorbic acid, and glutathione are presented and discussed. The results compared well with other standard methods for determination of superoxide dismutase, ascorbic acid and glutathione. Some possible steps in the overall reaction mechanisms are discussed.
英文摘要	This paper reports a new method for detection of ROS scavengers including superoxide dismutase, ascorbic acid and glutathione based on a 'probe' of peroxidase-oxidase biochemical oscillator. The oscillation period and amplitude change with different concentrations of scavengers. The linear ranges of superoxide dismutase, ascorbic acid and glutathione are respectively 1.56 x 10(-4)-1.56 x 10(-3) mg mL(-1), 1.75 x 10(-7) -1.75 x 10(-5) mol L-1 and 9.38 x 10(-7) -7.5 x 10(-5) mol L-1. The selectivity, linearity and precision for superoxide dismutase, ascorbic acid, and glutathione are presented and discussed. The results compared well with other standard methods for determination of superoxide dismutase, ascorbic acid and glutathione. Some possible steps in the overall reaction mechanisms are discussed.
学科主题	Chemistry ; Analytical
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	MECHANISM ; CHAOS
收录类别	SCI
语种	英语
WOS记录号	WOS:000181357900017
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9740]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Gan, NQ,Cai, RX,Lin, ZX,et al. Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator[J]. ANALYST,2003,128(3):301-305.
APA	Gan, NQ,Cai, RX,Lin, ZX,&Song, LR.(2003).Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator.ANALYST,128(3),301-305.
MLA	Gan, NQ,et al."Determination of some reactive oxygen species scavengers based on the peroxidase-oxidase oscillator".ANALYST 128.3(2003):301-305.

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