Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp

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	Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp
	Dai, HP ; Gao, H ; Zhao, XY ; Dai, LF ; Zhang, XK ; Xiao, N ; Zhao, RH ; Hemmingsen, SM
刊名	JOURNAL OF IMMUNOLOGICAL METHODS
	2003-08-01
卷号	279 期号:1-2 页码:267-275
关键词	phage display White Spot Syndrome Virus shrimp
ISSN号	0022-1759
通讯作者	Dai, HP, Chinese Acad Sci, Inst Hydrobiol, E Lake So Rd 7 Wuchan, Wuhan 430072, Hubei, Peoples R China
中文摘要	An antibody phage display library against White Spot Syndrome Virus (WSSV) was constructed. After four rounds of panning against WSSV, 192 out of 480 clones displayed WSSV binding activity. One of the positive clones, designated A1, had relatively higher activity specifically binding to WSSV A1-soluble, single-chain fragment variable (scFv) antibody has an affinity constant (K-aff) of 2.02 +/- 0.42 x 10(9) M-1. Dot blot assays showed that A1-soluble scFv could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV. A1 scFv has potential for the development of a cheap, simple and sensitive diagnostic kit for WSSV in the field. (C) 2003 Elsevier Science B.V. All rights reserved.
英文摘要	An antibody phage display library against White Spot Syndrome Virus (WSSV) was constructed. After four rounds of panning against WSSV, 192 out of 480 clones displayed WSSV binding activity. One of the positive clones, designated A1, had relatively higher activity specifically binding to WSSV A1-soluble, single-chain fragment variable (scFv) antibody has an affinity constant (K-aff) of 2.02 +/- 0.42 x 10(9) M-1. Dot blot assays showed that A1-soluble scFv could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV. A1 scFv has potential for the development of a cheap, simple and sensitive diagnostic kit for WSSV in the field. (C) 2003 Elsevier Science B.V. All rights reserved.
学科主题	Biochemical Research Methods; Immunology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Immunology
研究领域[WOS]	Biochemistry & Molecular Biology ; Immunology
关键词[WOS]	PHAGE DISPLAY ; GENOME SEQUENCE ; PENAEID SHRIMP ; BACULOVIRUS ; POLYMERASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000185381300024
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9650]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
推荐引用方式 GB/T 7714	Dai, HP,Gao, H,Zhao, XY,et al. Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2003,279(1-2):267-275.
APA	Dai, HP.,Gao, H.,Zhao, XY.,Dai, LF.,Zhang, XK.,...&Hemmingsen, SM.(2003).Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp.JOURNAL OF IMMUNOLOGICAL METHODS,279(1-2),267-275.
MLA	Dai, HP,et al."Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp".JOURNAL OF IMMUNOLOGICAL METHODS 279.1-2(2003):267-275.