Cloning of rainbow trout (Oncorhynchus mykiss) histone H3 promoter and the activity analysis in rare minnow (Gobiocypris rarus)

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	Cloning of rainbow trout (Oncorhynchus mykiss) histone H3 promoter and the activity analysis in rare minnow (Gobiocypris rarus)
	Mao, WF; Sun, YH; Wang, YP; Wu, G; Chen, SP; Zhu, ZY
刊名	PROGRESS IN NATURAL SCIENCE
	2004-04-01
卷号	14 期号:4 页码:322-+
关键词	enhanced green fluorescent protein (EGFP) histone H3 promoter transgenic fish rare minnow
ISSN号	1002-0071
通讯作者	Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Rainbow trout historic H3 (RH3) promoter was cloned via high fidelity PCR. The cloned RH3 promoter was inserted into a promoter-lacked vector pEGFP-1, resulting in an expression vector pRH3FGFP-1. The linearized pRH3EGFP-1 was microinjected into fertilized eggs of rare minnows and the sequential embryogenetic processes were monitored under a fluorescent microscope. Strong green fluorescence was ubiquitously observed at as early as the gastrula stage and then in various tissues at the fry stage. The results indicate that RH3 promoter, as a piscine promoter, could serve in producing transgenic Cyprinoid such as rare minnow. Promoter activity of RH3, CMV and common carp beta-actin (CA) were compared in rare minnow by the expression of respective recombinant EGFP vectors. The expression of pCMVEGFP occurred earlier than the following one, pRH3EGFP-1, and then pCAEGFP during the embryogenesis of the transgenics. Their expression activities demonstrated that the CMV promoter is the strongest one, followed by the CA and then the RH3.
英文摘要	Rainbow trout historic H3 (RH3) promoter was cloned via high fidelity PCR. The cloned RH3 promoter was inserted into a promoter-lacked vector pEGFP-1, resulting in an expression vector pRH3FGFP-1. The linearized pRH3EGFP-1 was microinjected into fertilized eggs of rare minnows and the sequential embryogenetic processes were monitored under a fluorescent microscope. Strong green fluorescence was ubiquitously observed at as early as the gastrula stage and then in various tissues at the fry stage. The results indicate that RH3 promoter, as a piscine promoter, could serve in producing transgenic Cyprinoid such as rare minnow. Promoter activity of RH3, CMV and common carp beta-actin (CA) were compared in rare minnow by the expression of respective recombinant EGFP vectors. The expression of pCMVEGFP occurred earlier than the following one, pRH3EGFP-1, and then pCAEGFP during the embryogenesis of the transgenics. Their expression activities demonstrated that the CMV promoter is the strongest one, followed by the CA and then the RH3.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology ; Technology
类目[WOS]	Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]	Materials Science ; Science & Technology - Other Topics
关键词[WOS]	ATLANTIC SALMON ; GENE ; FISH ; EXPRESSION ; RESISTANCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000220911000006
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9510]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Mao, WF,Sun, YH,Wang, YP,et al. Cloning of rainbow trout (Oncorhynchus mykiss) histone H3 promoter and the activity analysis in rare minnow (Gobiocypris rarus)[J]. PROGRESS IN NATURAL SCIENCE,2004,14(4):322-+.
APA	Mao, WF,Sun, YH,Wang, YP,Wu, G,Chen, SP,&Zhu, ZY.(2004).Cloning of rainbow trout (Oncorhynchus mykiss) histone H3 promoter and the activity analysis in rare minnow (Gobiocypris rarus).PROGRESS IN NATURAL SCIENCE,14(4),322-+.
MLA	Mao, WF,et al."Cloning of rainbow trout (Oncorhynchus mykiss) histone H3 promoter and the activity analysis in rare minnow (Gobiocypris rarus)".PROGRESS IN NATURAL SCIENCE 14.4(2004):322-+.