Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro
	Hong, YH ; Liu, TM ; Zhao, HB ; Xu, HY ; Wang, WJ ; Liu, R ; Chen, TS ; Deng, JR ; Gui, JF
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2004-05-25
卷号	101 期号:21 页码:8011-8016
关键词	germ stem cells meiosis Oryzias latipes spermiogenesis testis
ISSN号	0027-8424
通讯作者	Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore
中文摘要	Spermatogonia are the male germ stem cells that continuously produce sperm for the next generation. Spermatogenesis is a complicated process that proceeds through mitotic phase of stem cell renewal and differentiation, meiotic phase, and postmeiotic phase of spermiogenesis. Full recapitulation of spermatogenesis in vitro has been impossible, as generation of normal spermatogonial stem cell lines without immortalization and production of motile sperm from these cells after long-term culture have not been achieved. Here we report the derivation of a normal spermatogonial cell line from a mature medakafish testis without immortalization. After 140 passages during 2 years of culture, this cell line retains stable but growth factor-dependent proliferation, a diploid karyotype, and the phenotype and gene expression pattern of spermatogonial stem cells. Furthermore, we show that this cell line can undergo meiosis and spermiogenesis to generate motile sperm. Therefore, the ability of continuous proliferation and sperm production in culture is an intrinsic property of medaka spermatogonial stem cells, and immortalization apparently is not necessary to derive male germ cell cultures. Our findings and cell line will offer a unique opportunity to study and recapitulate spermatogenesis in vitro and to develop approaches for germ-line transmission.
英文摘要	Spermatogonia are the male germ stem cells that continuously produce sperm for the next generation. Spermatogenesis is a complicated process that proceeds through mitotic phase of stem cell renewal and differentiation, meiotic phase, and postmeiotic phase of spermiogenesis. Full recapitulation of spermatogenesis in vitro has been impossible, as generation of normal spermatogonial stem cell lines without immortalization and production of motile sperm from these cells after long-term culture have not been achieved. Here we report the derivation of a normal spermatogonial cell line from a mature medakafish testis without immortalization. After 140 passages during 2 years of culture, this cell line retains stable but growth factor-dependent proliferation, a diploid karyotype, and the phenotype and gene expression pattern of spermatogonial stem cells. Furthermore, we show that this cell line can undergo meiosis and spermiogenesis to generate motile sperm. Therefore, the ability of continuous proliferation and sperm production in culture is an intrinsic property of medaka spermatogonial stem cells, and immortalization apparently is not necessary to derive male germ cell cultures. Our findings and cell line will offer a unique opportunity to study and recapitulate spermatogenesis in vitro and to develop approaches for germ-line transmission.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	EMBRYONIC STEM-CELLS ; LONG-TERM CULTURE ; GERM-CELLS ; ORYZIAS-LATIPES ; GENE-TRANSFER ; MOUSE ; DIFFERENTIATION ; EXPRESSION ; TRANSPLANTATION ; SPERMATOGENESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000221652000032
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9478]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Hong, YH,Liu, TM,Zhao, HB,et al. Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2004,101(21):8011-8016.
APA	Hong, YH.,Liu, TM.,Zhao, HB.,Xu, HY.,Wang, WJ.,...&Gui, JF.(2004).Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,101(21),8011-8016.
MLA	Hong, YH,et al."Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101.21(2004):8011-8016.