An ELISA-like time-resolved fluorescence immunoassay for microcystin detection

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	An ELISA-like time-resolved fluorescence immunoassay for microcystin detection
	Lei, LM ; Wu, YS ; Gan, NQ ; Song, LR
刊名	CLINICA CHIMICA ACTA
	2004-10-01
卷号	348 期号:1-2 页码:177-180
关键词	time-resolved fluorescence immunoassay ELISA microcystin
ISSN号	0009-8981
通讯作者	Song, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
中文摘要	Background: A time-resolved fluorescence immunoassay (TRFIA), based on anti-microcystin-LR (MCLR) monoclonal antibodies (MAbs) and europium-labeled antimouse IgG conjugate, was first developed for microcystin detection. Methods: Anti-MCLR MAbs were prepared by a standard method, and the attained MAbs showed a good cross reactivity with MCLR, MCRR and MCYR. The TRFIA was performed in an indirect competitive mode. The detection method of TRFIA was compared with indirect competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). Results: The TRFIA exhibited a typical sigmoidal response for MCLR at concentrations of 0.005-50 ng/ml, with a wide quantitative range between 0.01 and 10 ng/ml, indicating the broadest detective range and the most sensitive of all the methods for microcystins (MCs) detection. Additionally, the TRFIA maintained good reliability through its quantitative range, as evidenced by low coefficients of variation (1.6-12.2%). The toxin data of algal samples assayed from TRFIA were in the same range as those with ELISA and HPLC, implying that the method was reliable and practical for the detection of MCs. Conclusions: The TRFIA may offer a valuable alternative or a substitute for conventional ELISA for microcystin detection. (C) 2004 Elsevier B.V. All rights reserved.
英文摘要	Background: A time-resolved fluorescence immunoassay (TRFIA), based on anti-microcystin-LR (MCLR) monoclonal antibodies (MAbs) and europium-labeled antimouse IgG conjugate, was first developed for microcystin detection. Methods: Anti-MCLR MAbs were prepared by a standard method, and the attained MAbs showed a good cross reactivity with MCLR, MCRR and MCYR. The TRFIA was performed in an indirect competitive mode. The detection method of TRFIA was compared with indirect competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). Results: The TRFIA exhibited a typical sigmoidal response for MCLR at concentrations of 0.005-50 ng/ml, with a wide quantitative range between 0.01 and 10 ng/ml, indicating the broadest detective range and the most sensitive of all the methods for microcystins (MCs) detection. Additionally, the TRFIA maintained good reliability through its quantitative range, as evidenced by low coefficients of variation (1.6-12.2%). The toxin data of algal samples assayed from TRFIA were in the same range as those with ELISA and HPLC, implying that the method was reliable and practical for the detection of MCs. Conclusions: The TRFIA may offer a valuable alternative or a substitute for conventional ELISA for microcystin detection. (C) 2004 Elsevier B.V. All rights reserved.
学科主题	Medical Laboratory Technology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Medical Laboratory Technology
研究领域[WOS]	Medical Laboratory Technology
关键词[WOS]	CYANOBACTERIAL TOXINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000224337100024
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9406]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Lei, LM,Wu, YS,Gan, NQ,et al. An ELISA-like time-resolved fluorescence immunoassay for microcystin detection[J]. CLINICA CHIMICA ACTA,2004,348(1-2):177-180.
APA	Lei, LM,Wu, YS,Gan, NQ,&Song, LR.(2004).An ELISA-like time-resolved fluorescence immunoassay for microcystin detection.CLINICA CHIMICA ACTA,348(1-2),177-180.
MLA	Lei, LM,et al."An ELISA-like time-resolved fluorescence immunoassay for microcystin detection".CLINICA CHIMICA ACTA 348.1-2(2004):177-180.