Characterization of development-related genes for the cestode Bothriocephalus acheilognathi

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Characterization of development-related genes for the cestode Bothriocephalus acheilognathi
	Luo, HY ; Nie, P ; Chang, MX ; Song, Y ; Yao, WJ
刊名	PARASITOLOGY RESEARCH
	2004-10-01
卷号	94 期号:4 页码:265-274
关键词	MUCIN-LIKE PROTEIN
ISSN号	0932-0113
通讯作者	Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Differential gene expression of mature and immature Bothriocephalus acheilognathi cestodes was analyzed using the suppression subtractive hybridization technique. Five mature-associated cDNAs were isolated and characterized. Virtual Northern blot and RT-PCR analyses confirmed that four of the five genes were up-regulated in mature parasites. The sequence analysis revealed that one gene encoded the structural protein chorion precursor, and that three encoded functional proteins homologous to yolk ferritin, sodium/hydrogen exchanger and muscin-like protein. Another gene appeared to be specific to B. acheilognathi, encoding a putative metal-bound protein. Although results obtained in the present study are preliminary, the information about the five genes may provide clues for further investigation on the decline in parasite numbers during the maturation of B. acheilognathi.
英文摘要	Differential gene expression of mature and immature Bothriocephalus acheilognathi cestodes was analyzed using the suppression subtractive hybridization technique. Five mature-associated cDNAs were isolated and characterized. Virtual Northern blot and RT-PCR analyses confirmed that four of the five genes were up-regulated in mature parasites. The sequence analysis revealed that one gene encoded the structural protein chorion precursor, and that three encoded functional proteins homologous to yolk ferritin, sodium/hydrogen exchanger and muscin-like protein. Another gene appeared to be specific to B. acheilognathi, encoding a putative metal-bound protein. Although results obtained in the present study are preliminary, the information about the five genes may provide clues for further investigation on the decline in parasite numbers during the maturation of B. acheilognathi.
学科主题	Parasitology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Parasitology
研究领域[WOS]	Parasitology
关键词[WOS]	MUCIN-LIKE PROTEIN ; SCHISTOSOMA-MANSONI ; FASCIOLA-HEPATICA ; CYPRINUS-CARPIO ; PRECURSOR ; GLYCOPROTEINS ; SEQUENCES ; PARASITE ; FERRITIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000224615200003
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9398]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Luo, HY,Nie, P,Chang, MX,et al. Characterization of development-related genes for the cestode Bothriocephalus acheilognathi[J]. PARASITOLOGY RESEARCH,2004,94(4):265-274.
APA	Luo, HY,Nie, P,Chang, MX,Song, Y,&Yao, WJ.(2004).Characterization of development-related genes for the cestode Bothriocephalus acheilognathi.PARASITOLOGY RESEARCH,94(4),265-274.
MLA	Luo, HY,et al."Characterization of development-related genes for the cestode Bothriocephalus acheilognathi".PARASITOLOGY RESEARCH 94.4(2004):265-274.