Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China)

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	Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China)
	Cao, XY ; Strojsova, A ; Znachor, P ; Zapomelova, E ; Liu, GX ; Vrba, J ; Zhou, YY
刊名	EUROPEAN JOURNAL OF PHYCOLOGY
	2005-08-01
卷号	40 期号:3 页码:251-258
关键词	enzyme-labelled fluorescence phosphatase activity phosphorus phytoplankton protozoa
ISSN号	0967-0262
通讯作者	Zhou, YY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	The species-specific production of extracellular phosphatases in phytoplankton of a subtropical polymictic take was investigated from March to May 2004. Phosphatase activity was detected directly at the site of enzyme action using the enzyme-labelled fluorescence (ELF) technique. Size fractionation of bulk phosphatase activity (PA), concentrations of soluble reactive phosphorus (SRP), chlorophyll a, and phytoplankton composition were determined in parallel. Phosphatase-positive cells were present in every phytoplankton sample; labelled cells were detected in 33 algal taxa, including many chlorophytes, dinoflagellates and some diatoms, but never among cyanobacteria. We recorded an unusual dinoflagellate bloom (Peridiniopsis sp.), of which similar to 25% of the cells were phosphatase-positive. Several populations were partly phosphatase-positive whenever present, while some other species never showed any activity. The production of extracellular phosphatases was not primarily regulated by ambient P concentrations; algae produced these enzymes even if SRP concentrations were high. Moreover, heterotrophic nanoflagellates most probably contributed to the pool of particle-bound PA in some samples.
英文摘要	The species-specific production of extracellular phosphatases in phytoplankton of a subtropical polymictic take was investigated from March to May 2004. Phosphatase activity was detected directly at the site of enzyme action using the enzyme-labelled fluorescence (ELF) technique. Size fractionation of bulk phosphatase activity (PA), concentrations of soluble reactive phosphorus (SRP), chlorophyll a, and phytoplankton composition were determined in parallel. Phosphatase-positive cells were present in every phytoplankton sample; labelled cells were detected in 33 algal taxa, including many chlorophytes, dinoflagellates and some diatoms, but never among cyanobacteria. We recorded an unusual dinoflagellate bloom (Peridiniopsis sp.), of which similar to 25% of the cells were phosphatase-positive. Several populations were partly phosphatase-positive whenever present, while some other species never showed any activity. The production of extracellular phosphatases was not primarily regulated by ambient P concentrations; algae produced these enzymes even if SRP concentrations were high. Moreover, heterotrophic nanoflagellates most probably contributed to the pool of particle-bound PA in some samples.
学科主题	Plant Sciences; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences ; Marine & Freshwater Biology
研究领域[WOS]	Plant Sciences ; Marine & Freshwater Biology
关键词[WOS]	DISSOLVED ORGANIC PHOSPHORUS ; ALKALINE-PHOSPHATASE ; WATER ; KINETICS ; SEDIMENTS ; QUANTIFICATION ; AVAILABILITY ; FLUORESCENCE ; DEFICIENCY ; INDICATORS
收录类别	SCI
语种	英语
WOS记录号	WOS:000232831200002
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9120]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Acad Sci Czech Republ, Inst Hydrobiol, CZ-37005 Ceske Budejovice, Czech Republic 4.Univ S Bohemia, Fac Biol Sci, CZ-37005 Ceske Budejovice, Czech Republic
推荐引用方式 GB/T 7714	Cao, XY,Strojsova, A,Znachor, P,et al. Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China)[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2005,40(3):251-258.
APA	Cao, XY.,Strojsova, A.,Znachor, P.,Zapomelova, E.,Liu, GX.,...&Zhou, YY.(2005).Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China).EUROPEAN JOURNAL OF PHYCOLOGY,40(3),251-258.
MLA	Cao, XY,et al."Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China)".EUROPEAN JOURNAL OF PHYCOLOGY 40.3(2005):251-258.