Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group

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	Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group
	Jin, S ; Hu, GA ; Qian, YH ; Zhang, L ; Zhang, J ; Qiu, G ; Zeng, QT ; Gui, JF
刊名	GENETICA
	2005-11-01
卷号	125 期号:2-3 页码:223-230
关键词	Dfak56 Drosophila melanogaster intron loss phylogenetic evolution single-copy nuclear gene
ISSN号	0016-6707
通讯作者	Gui, JF, Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China
中文摘要	Intron loss and its evolutionary significance have been noted in Drosophila. The current study provides another example of intron loss within a single-copy Dfak gene in Drosophila. By using polymerase chain reaction (PCR), we amplified about 1.3 kb fragment spanning intron 5-10, located in the position of Tyr kinase (TyK) domain of Dfak gene from Drosophila melanogaster species group, and observed size difference among the amplified DNA fragments from different species. Further sequencing analysis revealed that D. melanogaster and D. simulans deleted an about 60 bp of DNA fragment relative to other 7 Drosophila species, such as D. elegans, D. ficusphila, D. biarmipes, D. takahashii, D. jambulina, D. prostipennis and D. pseudoobscura, and the deleted fragment located precisely in the position of one intron. The data suggested that intron loss might have occurred in the Dfak gene evolutionary process of D. melanogaster and D. simulans of Drosophila melanogaster species group. In addition, the constructed phylogenetic tree based on the Dfak TyK domains clearly revealed the evolutionary relationships between subgroups of Drosophila melanogaster species group, and the intron loss identified from D. melanogaster and D. simulans provides a unique diagnostic tool for taxonomic classification of the melanogaster subgroup from other group of genus Drosophila.
英文摘要	Intron loss and its evolutionary significance have been noted in Drosophila. The current study provides another example of intron loss within a single-copy Dfak gene in Drosophila. By using polymerase chain reaction (PCR), we amplified about 1.3 kb fragment spanning intron 5-10, located in the position of Tyr kinase (TyK) domain of Dfak gene from Drosophila melanogaster species group, and observed size difference among the amplified DNA fragments from different species. Further sequencing analysis revealed that D. melanogaster and D. simulans deleted an about 60 bp of DNA fragment relative to other 7 Drosophila species, such as D. elegans, D. ficusphila, D. biarmipes, D. takahashii, D. jambulina, D. prostipennis and D. pseudoobscura, and the deleted fragment located precisely in the position of one intron. The data suggested that intron loss might have occurred in the Dfak gene evolutionary process of D. melanogaster and D. simulans of Drosophila melanogaster species group. In addition, the constructed phylogenetic tree based on the Dfak TyK domains clearly revealed the evolutionary relationships between subgroups of Drosophila melanogaster species group, and the intron loss identified from D. melanogaster and D. simulans provides a unique diagnostic tool for taxonomic classification of the melanogaster subgroup from other group of genus Drosophila.
学科主题	Genetics & Heredity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Genetics & Heredity
研究领域[WOS]	Genetics & Heredity
关键词[WOS]	FOCAL ADHESION KINASE ; MOLECULAR EVOLUTION ; CARASSIUS-AURATUS ; GAIN ; RNA ; POLYMORPHISM ; TRANSFERRIN ; SEQUENCES ; SELECTION ; INSERTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000232841800010
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9118]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Jin, S,Hu, GA,Qian, YH,et al. Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group[J]. GENETICA,2005,125(2-3):223-230.
APA	Jin, S.,Hu, GA.,Qian, YH.,Zhang, L.,Zhang, J.,...&Gui, JF.(2005).Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group.GENETICA,125(2-3),223-230.
MLA	Jin, S,et al."Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group".GENETICA 125.2-3(2005):223-230.