NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis

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	NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis
	Wang, Q ; Wang, YP ; Xu, P ; Liu, ZJ
刊名	MOLECULAR IMMUNOLOGY
	2006-04-01
卷号	43 期号:10 页码:1676-1686
关键词	antimicrobial peptide fish catfish disease NK-lysin innate immunity gene
ISSN号	0161-5890
通讯作者	Liu, ZJ, Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, 203 Swingle Hall, Auburn, AL 36849 USA
中文摘要	Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. In addition to the previously known four classes of antimicrobial peptides, a fifth class of antimicrobial peptides has been recently identified to include NK-lysins that have a globular three-dimensional structure and are larger with 74-78 amino acid residues. NK-lysin has been shown to harbor antimicrobial activities against a wide spectrum of microorganisms including bacteria, fungi, protozoa, and parasites. To date, NK-lysin genes have been reported from only a limited number of organisms. We previously identified a NK-lysin cDNA in channel catfish. Here we report the identification of two noveltypes of NK-lysin transcripts in channel catfish. Altogether, three distinct NK-lysin transcripts exist in channel catfish. In this work, their encoding genes were identified, sequenced, and characterized. We provide strong evidence that the catfish NK-lysin gene is tripled in the same genomic neighborhood. All three catfish NK-lysin genes are present in the same genomic region and are tightly linked on the same chromosome, as the same BAC clones harbor all three copies of the NK-lysin genes. All three NK-lysin genes are expressed, but exhibit distinct expression profiles in various tissues. In spite of the existence of a single copy of NK-lysin gene in the human genome, and only a single hit from the pufferfish,genome, there are two tripled clusters of NK-lysin genes on chromosome 17 of zebrafish in addition to one more copy on its chromosome 5. The similarity in the genomic arrangement of the tripled NK-lysin genes in channel catfish and zebrafish suggest similar evolution of NK-lysin genes. (c) 2005 Elsevier Ltd. All rights reserved.
英文摘要	Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. In addition to the previously known four classes of antimicrobial peptides, a fifth class of antimicrobial peptides has been recently identified to include NK-lysins that have a globular three-dimensional structure and are larger with 74-78 amino acid residues. NK-lysin has been shown to harbor antimicrobial activities against a wide spectrum of microorganisms including bacteria, fungi, protozoa, and parasites. To date, NK-lysin genes have been reported from only a limited number of organisms. We previously identified a NK-lysin cDNA in channel catfish. Here we report the identification of two noveltypes of NK-lysin transcripts in channel catfish. Altogether, three distinct NK-lysin transcripts exist in channel catfish. In this work, their encoding genes were identified, sequenced, and characterized. We provide strong evidence that the catfish NK-lysin gene is tripled in the same genomic neighborhood. All three catfish NK-lysin genes are present in the same genomic region and are tightly linked on the same chromosome, as the same BAC clones harbor all three copies of the NK-lysin genes. All three NK-lysin genes are expressed, but exhibit distinct expression profiles in various tissues. In spite of the existence of a single copy of NK-lysin gene in the human genome, and only a single hit from the pufferfish,genome, there are two tripled clusters of NK-lysin genes on chromosome 17 of zebrafish in addition to one more copy on its chromosome 5. The similarity in the genomic arrangement of the tripled NK-lysin genes in channel catfish and zebrafish suggest similar evolution of NK-lysin genes. (c) 2005 Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Immunology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Immunology
研究领域[WOS]	Biochemistry & Molecular Biology ; Immunology
关键词[WOS]	ANTIMICROBIAL PEPTIDE GENE ; T-CELL-ACTIVATION ; ICTALURUS-PUNCTATUS ; WINTER FLOUNDER ; DIFFERENTIAL EXPRESSION ; AMERICANUS WALBAUM ; GENOMIC STRUCTURE ; HEPCIDIN GENE ; CC CHEMOKINES ; TELEOST FISH
收录类别	SCI
语种	英语
WOS记录号	WOS:000236450900019
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9004]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA 2.Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA 3.E China Normal Univ, Shanghai 200062, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang, Q,Wang, YP,Xu, P,et al. NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis[J]. MOLECULAR IMMUNOLOGY,2006,43(10):1676-1686.
APA	Wang, Q,Wang, YP,Xu, P,&Liu, ZJ.(2006).NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis.MOLECULAR IMMUNOLOGY,43(10),1676-1686.
MLA	Wang, Q,et al."NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis".MOLECULAR IMMUNOLOGY 43.10(2006):1676-1686.