Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock

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	Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock
	Dong, CW; Zhang, YB; Zhang, QY; Gui, HF
刊名	FISH & SHELLFISH IMMUNOLOGY
	2006-08-01
卷号	21 期号:2 页码:146-158
关键词	heat shock protein (Hsp) 40 paralichthys olivaceus Scophthalmus maximus rhabdovirus differential expression heat shock
ISSN号	1050-4648
通讯作者	Gui, HF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Heat shock proteins (Hsps) are a family of highly conserved cellular proteins present in all organisms, mediating a range of essential housekeeping and cytoprotective functions as well-known molecular chaperons and recently as regulators of the immune response. By subtractive suppression hybridization, three Hsp40 homologues have been identified in the flounder (Paralichthys olivaceus) embryonic cells (FEC) after treatment with UV-inactivated turbot (Scophthalmus maximus L.) rhabdovirus (SMRV), termed PoHsp40A4, PoHsp40B6 and PoHsp40B11, whose encoded proteins all possess the conserved DnaJ domain, a signature motif of the Hsp40 family. Based on different protein structure and phylogenetic analysis, they can be categorized into two subfamilies, PoHsp40A4 for Type I Hsp40, PoHsp40B6 and PoHsp40B11 for Type 11 Hsp40. Further expression analysis revealed two very different types of kinetics in response either to heat shock or to virus infection, with a marked induction for PoHsp4OA4 and a weak one for both PoHsp40B6 and PoHsp40B11. A very distinct tissue distribution of mRNA was also revealed among the three genes, even between PoHsp40B6 and PoHsp40B11. This is the first report on the transcriptional induction of Hsp40 in virally stimulated fish cells, and the differential expressions might reflect their different roles in unstressed and stressed cells. (c) 2005 Elsevier Ltd. All rights reserved.
英文摘要	Heat shock proteins (Hsps) are a family of highly conserved cellular proteins present in all organisms, mediating a range of essential housekeeping and cytoprotective functions as well-known molecular chaperons and recently as regulators of the immune response. By subtractive suppression hybridization, three Hsp40 homologues have been identified in the flounder (Paralichthys olivaceus) embryonic cells (FEC) after treatment with UV-inactivated turbot (Scophthalmus maximus L.) rhabdovirus (SMRV), termed PoHsp40A4, PoHsp40B6 and PoHsp40B11, whose encoded proteins all possess the conserved DnaJ domain, a signature motif of the Hsp40 family. Based on different protein structure and phylogenetic analysis, they can be categorized into two subfamilies, PoHsp40A4 for Type I Hsp40, PoHsp40B6 and PoHsp40B11 for Type 11 Hsp40. Further expression analysis revealed two very different types of kinetics in response either to heat shock or to virus infection, with a marked induction for PoHsp4OA4 and a weak one for both PoHsp40B6 and PoHsp40B11. A very distinct tissue distribution of mRNA was also revealed among the three genes, even between PoHsp40B6 and PoHsp40B11. This is the first report on the transcriptional induction of Hsp40 in virally stimulated fish cells, and the differential expressions might reflect their different roles in unstressed and stressed cells. (c) 2005 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	INDUCED SIGNAL-TRANSDUCTION ; ESCHERICHIA-COLI DNAJ ; FINGER-LIKE DOMAIN ; MOLECULAR CHAPERONE ; RAINBOW-TROUT ; CELLULAR INHIBITOR ; DENDRITIC CELLS ; ATPASE ACTIVITY ; MESSENGER-RNA ; PROTEIN EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000237633600004
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8972]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Dong, CW,Zhang, YB,Zhang, QY,et al. Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock[J]. FISH & SHELLFISH IMMUNOLOGY,2006,21(2):146-158.
APA	Dong, CW,Zhang, YB,Zhang, QY,&Gui, HF.(2006).Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock.FISH & SHELLFISH IMMUNOLOGY,21(2),146-158.
MLA	Dong, CW,et al."Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock".FISH & SHELLFISH IMMUNOLOGY 21.2(2006):146-158.