Isolation and characterization of 18 polymorphic microsatellite markers in Chinese mandarin fish Siniperca chuatsi (Basilewsky)

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	Isolation and characterization of 18 polymorphic microsatellite markers in Chinese mandarin fish Siniperca chuatsi (Basilewsky)
	Zhang, Bin ; Li, Zhong-Jie ; Tong, Jin-Gou ; Liao, Xiao-Lin
刊名	MOLECULAR ECOLOGY NOTES
	2006-12-01
卷号	6 期号:4 页码:1216-1218
关键词	genomic library microsatellites Siniperca chuatsi
ISSN号	1471-8278
通讯作者	Li, ZJ, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Eighteen microsatellite markers were isolated and characterized using an enrichment protocol in the Chinese mandarin fish Siniperca chuatsi (Basilewsky), a commercially important piscivorous fish in China. Out of 48 pairs of primers designed, 18 loci exhibited polymorphism with three to six alleles (mean 4.4 alleles/locus) and average observed heterozygosity ranged from 0.633 to 0.833 (mean 0.748) in a test population from Dongting Lake of China. Except for two loci, all other 16 loci were in the Hardy-Weinberg equilibrium. These markers would be useful for such studies as population genetics, ecology and selective breeding of the Chinese mandarin fish in future.
英文摘要	Eighteen microsatellite markers were isolated and characterized using an enrichment protocol in the Chinese mandarin fish Siniperca chuatsi (Basilewsky), a commercially important piscivorous fish in China. Out of 48 pairs of primers designed, 18 loci exhibited polymorphism with three to six alleles (mean 4.4 alleles/locus) and average observed heterozygosity ranged from 0.633 to 0.833 (mean 0.748) in a test population from Dongting Lake of China. Except for two loci, all other 16 loci were in the Hardy-Weinberg equilibrium. These markers would be useful for such studies as population genetics, ecology and selective breeding of the Chinese mandarin fish in future.
学科主题	Biochemistry & Molecular Biology; Ecology; Evolutionary Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
关键词[WOS]	DNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000242193300079
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8774]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Bin,Li, Zhong-Jie,Tong, Jin-Gou,et al. Isolation and characterization of 18 polymorphic microsatellite markers in Chinese mandarin fish Siniperca chuatsi (Basilewsky)[J]. MOLECULAR ECOLOGY NOTES,2006,6(4):1216-1218.
APA	Zhang, Bin,Li, Zhong-Jie,Tong, Jin-Gou,&Liao, Xiao-Lin.(2006).Isolation and characterization of 18 polymorphic microsatellite markers in Chinese mandarin fish Siniperca chuatsi (Basilewsky).MOLECULAR ECOLOGY NOTES,6(4),1216-1218.
MLA	Zhang, Bin,et al."Isolation and characterization of 18 polymorphic microsatellite markers in Chinese mandarin fish Siniperca chuatsi (Basilewsky)".MOLECULAR ECOLOGY NOTES 6.4(2006):1216-1218.