Identification of a putative oocyte-specific small nuclear ribonucleoprotein polypeptide C in gibel carp

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Identification of a putative oocyte-specific small nuclear ribonucleoprotein polypeptide C in gibel carp
	Wang, Hong-Ying; Zhou, Li; Gui, Jian-Fang
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2007
卷号	146 期号:1 页码:47-52
关键词	SNRPC nucleolus oogenesis embryogenesis Carassius ouratus gibelio oocyte Western blot RT-PCR immunofluorescence localization
ISSN号	1096-4959
通讯作者	Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
中文摘要	Previous studies have demonstrated that germinal vesicle of amphibian oocyte contains small nuclear ribonucleoprotein polypeptide C (SNRPC). In this study, a putative member of SNRPC was identified from Carassius auratus gibelio oocyte cDNA library. Its full-length cDNA has an open reading frame of 201 nt for encoding a peptide of 66 an, a short 5'-UTR of 19 nt and a long 3'-UTR of 347 nt including a polyadenylation signal and poly- (A) tail, and the deduced amino acid sequence has 47% identity with the C-terminal of the zebrafish small nuclear ribonucleoprotein polypeptide C. Western blot analysis revealed its oocyte-specific expression. Immunofluorescence localization indicated that its gene product localized to numerous nucleoli within the oocytes and showed dynamic changes with the nucleoli during oocyte maturation. RT-PCR and Western blot analysis further revealed its constant presence in the oocytes and in the embryos until hatching. The data suggested that the newly identified CagOSNRPC might be a nucleolar protein. (c) 2006 Elsevier Inc. All rights reserved.
英文摘要	Previous studies have demonstrated that germinal vesicle of amphibian oocyte contains small nuclear ribonucleoprotein polypeptide C (SNRPC). In this study, a putative member of SNRPC was identified from Carassius auratus gibelio oocyte cDNA library. Its full-length cDNA has an open reading frame of 201 nt for encoding a peptide of 66 an, a short 5'-UTR of 19 nt and a long 3'-UTR of 347 nt including a polyadenylation signal and poly- (A) tail, and the deduced amino acid sequence has 47% identity with the C-terminal of the zebrafish small nuclear ribonucleoprotein polypeptide C. Western blot analysis revealed its oocyte-specific expression. Immunofluorescence localization indicated that its gene product localized to numerous nucleoli within the oocytes and showed dynamic changes with the nucleoli during oocyte maturation. RT-PCR and Western blot analysis further revealed its constant presence in the oocytes and in the embryos until hatching. The data suggested that the newly identified CagOSNRPC might be a nucleolar protein. (c) 2006 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Zoology
研究领域[WOS]	Biochemistry & Molecular Biology ; Zoology
关键词[WOS]	CARASSIUS-AURATUS-GIBELIO ; SILVER CRUCIAN CARP ; TELEOST FISH ; PROTEIN ; OOGENESIS ; BLOCH ; EXPRESSION ; MATURATION ; GRANULES ; FAMILY
收录类别	SCI
语种	英语
WOS记录号	WOS:000244623600006
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8680]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hong-Ying,Zhou, Li,Gui, Jian-Fang. Identification of a putative oocyte-specific small nuclear ribonucleoprotein polypeptide C in gibel carp[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2007,146(1):47-52.
APA	Wang, Hong-Ying,Zhou, Li,&Gui, Jian-Fang.(2007).Identification of a putative oocyte-specific small nuclear ribonucleoprotein polypeptide C in gibel carp.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,146(1),47-52.
MLA	Wang, Hong-Ying,et al."Identification of a putative oocyte-specific small nuclear ribonucleoprotein polypeptide C in gibel carp".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 146.1(2007):47-52.