Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression

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	Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression
	Wang, Na ; Sun, Yong-Hua ; Liu, Jing ; Wang, Ya-Ping ; Zhu, Zuo-Yan
刊名	DEVELOPMENT GENES AND EVOLUTION
	2007-04-01
卷号	217 期号:4 页码:299-305
关键词	common carp maternal expression phylogenetic analysis Sonic hedgehog
ISSN号	0949-944X
通讯作者	Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Sonic hedgehog (Shh), one of important homologous members of the hedgehog (Hh) family in vertebrates, encodes a signaling molecule that is involved in short- or long-range patterning processes during embryogenesis. In zebrafish, maternal activity of Hh was found to be contributing to the formation of primary motoneurons. However, we found that all of the known Hh members were not maternally expressed in zebrafish. In the present study, full-length cDNA of common carp (Cyprinus carpio) Shh (cShh) was gained by degenerate reverse-transcription PCR (RT-PCR) and rapid amplification of cDNA ends. Sequence comparison shows that cShh coding sequence shares 93.4% identity with zebrafish Shh coding sequence, and their corresponding protein sequences have 91.9% similarity. Comparative analysis of Shh genomic sequences and Hh protein sequences from different species revealed that the genomic structures of Hh are conserved from invertebrate to vertebrate. In contrast to zebrafish Shh, cShh transcripts were detectable from one-cell stage by RT-PCR analysis. Whole mount in situ hybridization verified the maternal expression of Shh in common carp, which is, to our knowledge, the first report of that in vertebrates, suggesting that Shh might be responsible for the maternal Hh activity in common carp.
英文摘要	Sonic hedgehog (Shh), one of important homologous members of the hedgehog (Hh) family in vertebrates, encodes a signaling molecule that is involved in short- or long-range patterning processes during embryogenesis. In zebrafish, maternal activity of Hh was found to be contributing to the formation of primary motoneurons. However, we found that all of the known Hh members were not maternally expressed in zebrafish. In the present study, full-length cDNA of common carp (Cyprinus carpio) Shh (cShh) was gained by degenerate reverse-transcription PCR (RT-PCR) and rapid amplification of cDNA ends. Sequence comparison shows that cShh coding sequence shares 93.4% identity with zebrafish Shh coding sequence, and their corresponding protein sequences have 91.9% similarity. Comparative analysis of Shh genomic sequences and Hh protein sequences from different species revealed that the genomic structures of Hh are conserved from invertebrate to vertebrate. In contrast to zebrafish Shh, cShh transcripts were detectable from one-cell stage by RT-PCR analysis. Whole mount in situ hybridization verified the maternal expression of Shh in common carp, which is, to our knowledge, the first report of that in vertebrates, suggesting that Shh might be responsible for the maternal Hh activity in common carp.
学科主题	Cell Biology ; Evolutionary Biology ; Developmental Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Cell Biology ; Evolutionary Biology ; Developmental Biology
研究领域[WOS]	Cell Biology ; Evolutionary Biology ; Developmental Biology
关键词[WOS]	ZEBRAFISH ; GENE ; EMBRYOS
收录类别	SCI
语种	英语
WOS记录号	WOS:000245293600005
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8648]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Na,Sun, Yong-Hua,Liu, Jing,et al. Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression[J]. DEVELOPMENT GENES AND EVOLUTION,2007,217(4):299-305.
APA	Wang, Na,Sun, Yong-Hua,Liu, Jing,Wang, Ya-Ping,&Zhu, Zuo-Yan.(2007).Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression.DEVELOPMENT GENES AND EVOLUTION,217(4),299-305.
MLA	Wang, Na,et al."Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression".DEVELOPMENT GENES AND EVOLUTION 217.4(2007):299-305.