Fish ES cells and applications to biotechnology

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Fish ES cells and applications to biotechnology
	Alvarez, M. Carmen ; Bejar, Julia ; Chen, Songlin ; Hong, Yunhan
刊名	MARINE BIOTECHNOLOGY
	2007-03-01
卷号	9 期号:2 页码:117-127
关键词	chimeras ES cells fish transgenics
ISSN号	1436-2228
通讯作者	Alvarez, MC, Univ Malaga, Fac Sci, Dept Cell Biol & Genet, E-29071 Malaga, Spain
中文摘要	ES cells provide a promising tool for the generation of transgenic animals with site-directed mutations. When ES cells colonize germ cells in chimeras, transgenic animals with modified phenotypes are generated and used either for functional genomics studies or for improving productivity in commercial settings. Althought the ES cell approach has been limited to, mice, there is strong interest for developing the technology in fish.. We describe the step-by-step procedure for developing ES cells in fish. Key aspects include avoiding cell differentiation, specific in vitro traits of pluripotency, and, most importantly, testing for production of chimeric animals as the main evidence of pluripotency. The entire process focuses on two model species, zebrafish and medaka, in which most work has been done. The achievements attained in these species, as well as their applicability to other commercial fish, are discussed. Because of the difficulties relating to germ line competence, mostly of long-term fish ES cells, alternative cell-based approaches such as primordial germ cells and nuclear transfer need to be considered. Although progress to date has been slow, there are promising achievements in homologous recombination and alternative avenues yet to be explored that can bring ES technology in fish to fruition.
英文摘要	ES cells provide a promising tool for the generation of transgenic animals with site-directed mutations. When ES cells colonize germ cells in chimeras, transgenic animals with modified phenotypes are generated and used either for functional genomics studies or for improving productivity in commercial settings. Althought the ES cell approach has been limited to, mice, there is strong interest for developing the technology in fish.. We describe the step-by-step procedure for developing ES cells in fish. Key aspects include avoiding cell differentiation, specific in vitro traits of pluripotency, and, most importantly, testing for production of chimeric animals as the main evidence of pluripotency. The entire process focuses on two model species, zebrafish and medaka, in which most work has been done. The achievements attained in these species, as well as their applicability to other commercial fish, are discussed. Because of the difficulties relating to germ line competence, mostly of long-term fish ES cells, alternative cell-based approaches such as primordial germ cells and nuclear transfer need to be considered. Although progress to date has been slow, there are promising achievements in homologous recombination and alternative avenues yet to be explored that can bring ES technology in fish to fruition.
学科主题	Biotechnology & Applied Microbiology; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]	EMBRYONIC STEM-CELLS ; MEDAKA ORYZIAS-LATIPES ; GERM-LINE CHIMERAS ; GREEN FLUORESCENT PROTEIN ; EARLY ZEBRAFISH EMBRYOS ; VASA-LIKE GENE ; IN-VITRO ; TELOMERASE ACTIVITY ; HOMOLOGOUS RECOMBINATION ; HUMAN BLASTOCYSTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000245949800001
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8612]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Univ Malaga, Fac Sci, Dept Cell Biol & Genet, E-29071 Malaga, Spain 2.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 4.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
推荐引用方式 GB/T 7714	Alvarez, M. Carmen,Bejar, Julia,Chen, Songlin,et al. Fish ES cells and applications to biotechnology[J]. MARINE BIOTECHNOLOGY,2007,9(2):117-127.
APA	Alvarez, M. Carmen,Bejar, Julia,Chen, Songlin,&Hong, Yunhan.(2007).Fish ES cells and applications to biotechnology.MARINE BIOTECHNOLOGY,9(2),117-127.
MLA	Alvarez, M. Carmen,et al."Fish ES cells and applications to biotechnology".MARINE BIOTECHNOLOGY 9.2(2007):117-127.