Effects of generated trans-arachidonic acids on retinal capillary during nitrative stress in diabetic rats

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	Effects of generated trans-arachidonic acids on retinal capillary during nitrative stress in diabetic rats
	Xu L(许琳) ; Xun GL(寻国良) ; Yao ZJ(姚祝军) ; Liu YM(刘玉敏) ; Qiu YP(邱云平) ; Liu K(刘堃) ; Zhu DQ(朱冬青) ; Gu Q(顾青) ; Xu X(许迅) ; He ZP(何志平)
刊名	Ophthalmologica
	2008
卷号	222 期号:1 页码:37-41
ISSN号	0030-3755
其他题名	硝化应激中反式花生四烯酸聚集对糖尿病大鼠视网膜微血管的影响
通讯作者	许迅
英文摘要	Purpose: To investigate whether the trans-arachidonic acids (TAAs) change in diabetic conditions and the effect on capillary cells and its possible mechanism. Methods: Diabetes was induced by intraperitoneal injection of streptozotocin at 60 mg kg(-1) body weight. The amount of TAAs and arachidonic acid (AA) in serum of diabetic rats was measured by the gas chromatography and mass spectrometry method and the ratio of the peak area of TAAs to AA with a selected ion of 79 was estimated by group t test. The expression level of thrombospondin-1 (TSP-1) in the retina was examined by Western blot. The pericyte apoptotic index was investigated by flow cytometry. Result: The ratio of TAAs to AA significantly increased in serum at the time points of 8, 12 and 16 weeks after streptozotocin induction (p < 0.05). The expression of TSP-1 in the retina of diabetic rats elevated with longer duration of diabetes (checking the time points of 2-, 4-, 8-, 12- and 16-week diabetic rats). The expression of TSP-1 tended to be physiologically elevated in relation to age but less significantly than in the diabetic group. TAAs could not induce the apoptosis of retinal pericytes (p > 0.05). Conclusion: TAAs were generated associated with nitrative stress in diabetic rats and a possible mechanism of microvascular injury exits in diabetic conditions. Copyright (C) 2007 S. Karger AG, Basel.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1159/000109277
语种	英语
WOS记录号	WOS:000251835400008
公开日期	2013-02-26
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/21119]
专题	上海有机化学研究所_生命有机化学国家重点实验室
推荐引用方式 GB/T 7714	Xu L,Xun GL,Yao ZJ,et al. Effects of generated trans-arachidonic acids on retinal capillary during nitrative stress in diabetic rats[J]. Ophthalmologica,2008,222(1):37-41.
APA	许琳.,寻国良.,姚祝军.,刘玉敏.,邱云平.,...&何志平.(2008).Effects of generated trans-arachidonic acids on retinal capillary during nitrative stress in diabetic rats.Ophthalmologica,222(1),37-41.
MLA	许琳,et al."Effects of generated trans-arachidonic acids on retinal capillary during nitrative stress in diabetic rats".Ophthalmologica 222.1(2008):37-41.