Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)

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	Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)
	Zhou, L.; Li, C. J.; Wang, Y.; Xia, W.; Yao, B.; Jin, J. Y.; Gui, J. F.
刊名	JOURNAL OF CHEMICAL NEUROANATOMY
	2007-09-01
卷号	34 期号:1-2 页码:47-59
关键词	myelin basic protein orange-spotted grouper hypothalamus oligodendrocyte
ISSN号	0891-0618
通讯作者	Gui, JF, Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
中文摘要	Myelin basic protein (MBP), as a major component of the myelin sheath, has been revealed to play an important role informing and maintaining myelin structure in vertebrate nervous system. In teleost, hypothalamus is an instinctive brain center and plays significant roles in many physiological functions, such as energy metabolism, growth, reproduction, and stress response. In comparison with other MBP identified in vertebrates, a smallest MBP is cloned and identified from the orange-spotted grouper hypothalamic cDNA plasmid library in this study. RT-PCR analysis and Western blot detection indicate that the EcMBP is specific to hypothalamus, and expresses mainly in the tuberal hypothalamus in adult grouper. Immunofluorescence localization suggests that EcMBP should be expressed by oligodendrocytes, and the expressing cells should be concentrated in hypothalamus and the area surrounding hypothalamus, such as NPOpc, VC, DP, NLTm, and NDLI The studies on EcMBP expression pattern and developmental behaviour in the brains of grouper embryos and larvae reveal that the EcMBP-expressing cells are only limited in a defined set of cells on the border of hypothalamus, and suggest that the EcMBP-expressing cells might be a subpopulation of oliaodendrocyte progenitor cells. This study not only identifies a smallest MBP isoform specific to hypothalamus that can be used as a molecular marker of oligodendrocytes in fish, but also provides new insights for MBP evolution and cellular distribution. (C) 2007 Elsevier B.V.. All rights reserved.
英文摘要	Myelin basic protein (MBP), as a major component of the myelin sheath, has been revealed to play an important role informing and maintaining myelin structure in vertebrate nervous system. In teleost, hypothalamus is an instinctive brain center and plays significant roles in many physiological functions, such as energy metabolism, growth, reproduction, and stress response. In comparison with other MBP identified in vertebrates, a smallest MBP is cloned and identified from the orange-spotted grouper hypothalamic cDNA plasmid library in this study. RT-PCR analysis and Western blot detection indicate that the EcMBP is specific to hypothalamus, and expresses mainly in the tuberal hypothalamus in adult grouper. Immunofluorescence localization suggests that EcMBP should be expressed by oligodendrocytes, and the expressing cells should be concentrated in hypothalamus and the area surrounding hypothalamus, such as NPOpc, VC, DP, NLTm, and NDLI The studies on EcMBP expression pattern and developmental behaviour in the brains of grouper embryos and larvae reveal that the EcMBP-expressing cells are only limited in a defined set of cells on the border of hypothalamus, and suggest that the EcMBP-expressing cells might be a subpopulation of oliaodendrocyte progenitor cells. This study not only identifies a smallest MBP isoform specific to hypothalamus that can be used as a molecular marker of oligodendrocytes in fish, but also provides new insights for MBP evolution and cellular distribution. (C) 2007 Elsevier B.V.. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Neurosciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]	Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]	MYELIN-BASIC-PROTEIN ; RAT PANCREATIC-ISLETS ; GONADOTROPIN-RELEASING-HORMONE ; CENTRAL-NERVOUS-SYSTEM ; MOLECULAR-CLONING ; GOLDFISH BRAIN ; SEX REVERSAL ; OLIGODENDROCYTE SPECIFICATION ; DIFFERENTIAL EXPRESSION ; SCYLIORHINUS-TORAZAME
收录类别	SCI
语种	英语
WOS记录号	WOS:000248494100004
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8434]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhou, L.,Li, C. J.,Wang, Y.,et al. Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)[J]. JOURNAL OF CHEMICAL NEUROANATOMY,2007,34(1-2):47-59.
APA	Zhou, L..,Li, C. J..,Wang, Y..,Xia, W..,Yao, B..,...&Gui, J. F..(2007).Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides).JOURNAL OF CHEMICAL NEUROANATOMY,34(1-2),47-59.
MLA	Zhou, L.,et al."Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)".JOURNAL OF CHEMICAL NEUROANATOMY 34.1-2(2007):47-59.