Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8 beta and CD4-like genes

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	Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8 beta and CD4-like genes
	Sun, Xiao-Feng ; Shang, Na ; Hu, Wei ; Wang, Ya-Ping ; Guo, Qiong-Lin
刊名	FISH & SHELLFISH IMMUNOLOGY
	2007-12-01
卷号	23 期号:6 页码:1242-1255
关键词	CD8 beta CD4 T cells cell surface molecule common carp
ISSN号	1050-4648
通讯作者	Guo, QL, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Partial cDNA sequences of both CD8 beta and CD4-like (CD4L) genes of common carp (Cyprinus carpio L.) were isolated from thymus cDNA library by the method of suppression subtractive hybridization (SSH). Subsequently the full length cDNAs of carp CD8 and CD4L were obtained by means of 3' RACE and 5' RACE, respectively. The full length cDNA of carp CD8 is 1164 bp and encodes 207 amino acids including a signal peptide region of 24 amino acids, a transmembrane region of 23 amino acids from aa 167 to aa189 and an immunoglobulin V-set from aa 19 to aa 141. Similar to other species CD8 beta s,carp CD8 beta also lacks p56(lck) domain in the cytoplasmic region. The full length cDNA of carp CD4L is 2001 bp and encodes 458 amino acids including four immunoglobulin (Ig)-like domains in the extracellular region, a transmembrane region of 23 amino acids at the C-terminal region from aa 402 to aa 424 and a cytoplasmic tail. Similar to mammalian, avian CD4s and fugu CD4L, carp CD4L also has the conserved p56(lck) tyrosine kinase motif (C-X-C) in the cytoplasmic region. RT-PCR analysis demonstrated that carp CD8 beta and CD4L genes were both expressed predominantly in thymus. The results from this study can be used to understand the evolution of both the CD8 beta and CD4 molecules which can be used as markers for cytotoxic and helper T cells in carp. (c) 2007 Published by Elsevier Ltd.
英文摘要	Partial cDNA sequences of both CD8 beta and CD4-like (CD4L) genes of common carp (Cyprinus carpio L.) were isolated from thymus cDNA library by the method of suppression subtractive hybridization (SSH). Subsequently the full length cDNAs of carp CD8 and CD4L were obtained by means of 3' RACE and 5' RACE, respectively. The full length cDNA of carp CD8 is 1164 bp and encodes 207 amino acids including a signal peptide region of 24 amino acids, a transmembrane region of 23 amino acids from aa 167 to aa189 and an immunoglobulin V-set from aa 19 to aa 141. Similar to other species CD8 beta s,carp CD8 beta also lacks p56(lck) domain in the cytoplasmic region. The full length cDNA of carp CD4L is 2001 bp and encodes 458 amino acids including four immunoglobulin (Ig)-like domains in the extracellular region, a transmembrane region of 23 amino acids at the C-terminal region from aa 402 to aa 424 and a cytoplasmic tail. Similar to mammalian, avian CD4s and fugu CD4L, carp CD4L also has the conserved p56(lck) tyrosine kinase motif (C-X-C) in the cytoplasmic region. RT-PCR analysis demonstrated that carp CD8 beta and CD4L genes were both expressed predominantly in thymus. The results from this study can be used to understand the evolution of both the CD8 beta and CD4 molecules which can be used as markers for cytotoxic and helper T cells in carp. (c) 2007 Published by Elsevier Ltd.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	CLASS-II MHC ; HUMAN-IMMUNODEFICIENCY-VIRUS ; CD8 BETA-CHAIN ; HISTOCOMPATIBILITY COMPLEX-MOLECULES ; CARASSIUS-AURATUS-LANGSDORFII ; BASS DICENTRARCHUS-LABRAX ; GINBUNA CRUCIAN CARP ; T-CELLS ; CORECEPTOR FUNCTION ; ANTIGEN RECEPTOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000251472700010
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8340]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Sun, Xiao-Feng,Shang, Na,Hu, Wei,et al. Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8 beta and CD4-like genes[J]. FISH & SHELLFISH IMMUNOLOGY,2007,23(6):1242-1255.
APA	Sun, Xiao-Feng,Shang, Na,Hu, Wei,Wang, Ya-Ping,&Guo, Qiong-Lin.(2007).Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8 beta and CD4-like genes.FISH & SHELLFISH IMMUNOLOGY,23(6),1242-1255.
MLA	Sun, Xiao-Feng,et al."Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8 beta and CD4-like genes".FISH & SHELLFISH IMMUNOLOGY 23.6(2007):1242-1255.