TRAIL in the mandarin fish Siniperca chuatsi: Gene and its apoptotic effect in HeLa cells

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	TRAIL in the mandarin fish Siniperca chuatsi: Gene and its apoptotic effect in HeLa cells
	Gao, Y.1,2,3; Chang, M. X.1,2; Sun, B. J.1,2; Nie, P.1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2008
卷号	24 期号:1 页码:55-66
关键词	mandarin fish Siniperca chuatsi TRAIL apoptosis overexpression HeLa cells
ISSN号	1050-4648
通讯作者	Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
中文摘要	Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is one of the TNF superfamily members, participating in many biological processes including cell proliferation and apoptotic death. In this study, a TRAIL gene was cloned from a perciform fish, the mandarin fish Siniperca chuatsi, a major cultured fish in China's aquaculture, and is named as SCTRAIL for S. chuatsi TRAIL. The full-length cDNA of SCTRAIL is 1359 bp, encoding a 283-amino-acid protein. This deduced protein contains the CYS231, a 23-mer fragment of transmembrane region, a glycosylation site and a TNF family signature, all of which are conserved among TRAIL members. SCTRAIL gene consists of six exons, with five intervening introns, spaced over approximately 9 kb of genomic sequence. Southern blotting demonstrated that the SCTRAIL gene is present as a single copy in mandarin fish genome. A 620 bp promoter region obtained by genome walking contains a number of putative transcription factor binding sites, such as Oct-1, Sp-1, NF-1, RAP-1, C/EBPaLp, NF-kappa B and AP-1. The SCTRAIL is constitutively expressed in all the analyzed tissues, as revealed by RT-PCR, which is confirmed by Western blotting analysis using polyclonal antibody against bacteria-derived recombinant SCTRAIL protein. As an apoptosis-inducing ligand, the overexpression of SCTRAIL but not the mutant SCTRAIL-C203S in HeLa cells induced changes characteristic of apoptosis, including chromatin condensation, nucleus fragmentation, DNA ladder, and increase of sub-G0/G1 cells in FACS analysis. (c) 2007 Elsevier Ltd. All rights reserved.
英文摘要	Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is one of the TNF superfamily members, participating in many biological processes including cell proliferation and apoptotic death. In this study, a TRAIL gene was cloned from a perciform fish, the mandarin fish Siniperca chuatsi, a major cultured fish in China's aquaculture, and is named as SCTRAIL for S. chuatsi TRAIL. The full-length cDNA of SCTRAIL is 1359 bp, encoding a 283-amino-acid protein. This deduced protein contains the CYS231, a 23-mer fragment of transmembrane region, a glycosylation site and a TNF family signature, all of which are conserved among TRAIL members. SCTRAIL gene consists of six exons, with five intervening introns, spaced over approximately 9 kb of genomic sequence. Southern blotting demonstrated that the SCTRAIL gene is present as a single copy in mandarin fish genome. A 620 bp promoter region obtained by genome walking contains a number of putative transcription factor binding sites, such as Oct-1, Sp-1, NF-1, RAP-1, C/EBPaLp, NF-kappa B and AP-1. The SCTRAIL is constitutively expressed in all the analyzed tissues, as revealed by RT-PCR, which is confirmed by Western blotting analysis using polyclonal antibody against bacteria-derived recombinant SCTRAIL protein. As an apoptosis-inducing ligand, the overexpression of SCTRAIL but not the mutant SCTRAIL-C203S in HeLa cells induced changes characteristic of apoptosis, including chromatin condensation, nucleus fragmentation, DNA ladder, and increase of sub-G0/G1 cells in FACS analysis. (c) 2007 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	NECROSIS-FACTOR-ALPHA ; MOLECULAR-CLONING ; LIGAND TRAIL ; TRANSCRIPTIONAL REGULATION ; EXPRESSION ANALYSIS ; RECEPTOR ; REGION ; CDNA ; IDENTIFICATION ; INDUCTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000253306700007
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8266]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 10039, Peoples R China
推荐引用方式 GB/T 7714	Gao, Y.,Chang, M. X.,Sun, B. J.,et al. TRAIL in the mandarin fish Siniperca chuatsi: Gene and its apoptotic effect in HeLa cells[J]. FISH & SHELLFISH IMMUNOLOGY,2008,24(1):55-66.
APA	Gao, Y.,Chang, M. X.,Sun, B. J.,&Nie, P..(2008).TRAIL in the mandarin fish Siniperca chuatsi: Gene and its apoptotic effect in HeLa cells.FISH & SHELLFISH IMMUNOLOGY,24(1),55-66.
MLA	Gao, Y.,et al."TRAIL in the mandarin fish Siniperca chuatsi: Gene and its apoptotic effect in HeLa cells".FISH & SHELLFISH IMMUNOLOGY 24.1(2008):55-66.