Deletion of yeast CWP genes enhances cell permeability to genotoxic agents

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	Deletion of yeast CWP genes enhances cell permeability to genotoxic agents
	Zhang, Min 1; Liang, Yuping 2; Zhang, Xiaohua 1; Xu, Ying 1; Dai, Heping 1; Xiao, Wei 1,2
刊名	TOXICOLOGICAL SCIENCES
	2008-05-01
卷号	103 期号:1 页码:68-76
关键词	RNR3-lacZ yeast cell wall mannoprotein genotoxicity test sensitivity permeability
ISSN号	1096-6080
通讯作者	Dai, HP, Chinese Acad Sci, Inst Hydrol, 7 Donghu Rd S, Wuhan 430072, Peoples R China
中文摘要	We have previously reported the development of a novel genotoxic testing system based on the transcriptional response of the yeast RNR3-lacZ reporter gene to DNA damage. This system appears to be more sensitive than other similar tests in microorganisms, and is comparable with the Ames test. In an effort to further enhance detection sensitivity, we examined the effects of altering major cell wall components on cell permeability and subsequent RNR3-lacZ sensitivity to genotoxic agents. Although inactivation of single CWP genes encoding cell wall mannoproteins had little effect, the simultaneous inactivation of both CWP1 and CWP2 had profound effects on the cell wall structure and permeability. Consequently, the RNR3-lacZ detection sensitivity is markedly enhanced, especially to high molecular weight compounds such as 4-nitroquinoline-N-oxide (> sevenfold) and phleomycin (> 13-fold). In contrast, deletion of genes encoding representative membrane components or membrane transporters had minor effects on cell permeability. We conclude that the yeast cell wall mannoproteins constitute the major barrier to environmental genotoxic agents and that their removal will significantly enhance the sensitivity of RNR-lacZ as well as other yeast-based genotoxic tests.
英文摘要	We have previously reported the development of a novel genotoxic testing system based on the transcriptional response of the yeast RNR3-lacZ reporter gene to DNA damage. This system appears to be more sensitive than other similar tests in microorganisms, and is comparable with the Ames test. In an effort to further enhance detection sensitivity, we examined the effects of altering major cell wall components on cell permeability and subsequent RNR3-lacZ sensitivity to genotoxic agents. Although inactivation of single CWP genes encoding cell wall mannoproteins had little effect, the simultaneous inactivation of both CWP1 and CWP2 had profound effects on the cell wall structure and permeability. Consequently, the RNR3-lacZ detection sensitivity is markedly enhanced, especially to high molecular weight compounds such as 4-nitroquinoline-N-oxide (> sevenfold) and phleomycin (> 13-fold). In contrast, deletion of genes encoding representative membrane components or membrane transporters had minor effects on cell permeability. We conclude that the yeast cell wall mannoproteins constitute the major barrier to environmental genotoxic agents and that their removal will significantly enhance the sensitivity of RNR-lacZ as well as other yeast-based genotoxic tests.
学科主题	Toxicology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Toxicology
研究领域[WOS]	Toxicology
关键词[WOS]	PLEIOTROPIC DRUG-RESISTANCE ; SACCHAROMYCES-CEREVISIAE ; MULTIDRUG-RESISTANCE ; CALCOFLUOR WHITE ; TESTING SYSTEM ; WALL POROSITY ; CONGO RED ; MEMBRANE ; MANNOPROTEINS ; SENSITIVITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000254955500008
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8160]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrol, Wuhan 430072, Peoples R China 2.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
推荐引用方式 GB/T 7714	Zhang, Min,Liang, Yuping,Zhang, Xiaohua,et al. Deletion of yeast CWP genes enhances cell permeability to genotoxic agents[J]. TOXICOLOGICAL SCIENCES,2008,103(1):68-76.
APA	Zhang, Min,Liang, Yuping,Zhang, Xiaohua,Xu, Ying,Dai, Heping,&Xiao, Wei.(2008).Deletion of yeast CWP genes enhances cell permeability to genotoxic agents.TOXICOLOGICAL SCIENCES,103(1),68-76.
MLA	Zhang, Min,et al."Deletion of yeast CWP genes enhances cell permeability to genotoxic agents".TOXICOLOGICAL SCIENCES 103.1(2008):68-76.